Oxygen signal

high-resolution terminology - matching measurements at high-resolution

Oxygen signal

Description

[[Description::The oxygen signal of the O2k is transmitted from the electrochemical polarographic oxygen sensor (OroboPOS) for each of the two chambers to DatLab. The primary signal is a current mAmp which is converted into a voltage [V], and calibrated in SI units for amount of substrance concentration [µmol.dm-3 or µM].]]

Reference: [[Info::MiPNet19.18D O2k-Calibration, MiPNet06.03 POS-Calibration-SOP]]

MitoPedia methods: Respirometry, DatLab 

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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 

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Stability of the oxygen signal

The stability of the oxygen signal of the O2k is evaluated by the (negative) uncorrected slope over time. After smoothing, the slope of a stable sensor should be less than +- 1 pmol.s-1.ml-1 in a 2-ml chamber.

• Instability of the oxygen signal

• There is drift of the oxygen signal over short or long periods of time.
• The signal at air saturation is abnormally high for a given gain setting.
• The zero current is high (>2.5%) and may show drift.

• Confirm and locate the problem by performing a Sensor test and following Locating a problem.

Follow the standard instructions for oxygen sensor service.

= Oxygen signal does not respond

• The oxygen signal remains at or close to zero even at high oxygen levels.
• The signal at air saturation is abnormally low.

1. Check the gain setting for the affected oxygen channel.

• Response 1: The gain setting was too low, and the oxygen signal increases to the expected level after increasing the gain.
• Response 2: The gain was correct: Locate the problem using the procedure described in Locating a problem and Sensor test.