Ebanks 2023 PLoS One: Difference between revisions
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|title=Ebanks B, Kwiecinska P, Moisoi N, Chakrabarti L (2023) A method to assess the mitochondrial respiratory capacity of complexes I and II from frozen tissue using the Oroboros O2k-FluoRespirometer. https://doi.org/10.1371/journal.pone.0276147 | |title=Ebanks B, Kwiecinska P, Moisoi N, Chakrabarti L (2023) A method to assess the mitochondrial respiratory capacity of complexes I and II from frozen tissue using the Oroboros O2k-FluoRespirometer. https://doi.org/10.1371/journal.pone.0276147 | ||
|info=PLoS One 18:e0276147. [https://www.ncbi.nlm.nih.gov/pubmed/37486925 PMID: 37486925 Open Access] | |info=PLoS One 18:e0276147. [https://www.ncbi.nlm.nih.gov/pubmed/37486925 PMID: 37486925 Open Access] | ||
|authors=Ebanks | |authors=Ebanks Brad, Kwiecinska Pola, Moisoi Nicoleta, Chakrabarti Lisa | ||
|year=2023 | |year=2023 | ||
|journal=PLoS One | |journal=PLoS One | ||
|abstract=High-resolution respirometry methods allow for the assessment of oxygen consumption by the electron transfer systems within cells, tissue samples, and isolated mitochondrial preparations. As mitochondrial integrity is compromised by the process of cryopreservation, these methods have been limited to fresh samples. Here we present a simple method to assess the activity of mitochondria respiratory complexes I and II in previously cryopreserved murine skeletal muscle tissue homogenates, as well as previously frozen D. melanogaster, as a function of oxygen consumption. | |abstract=High-resolution respirometry methods allow for the assessment of oxygen consumption by the electron transfer systems within cells, tissue samples, and isolated mitochondrial preparations. As mitochondrial integrity is compromised by the process of cryopreservation, these methods have been limited to fresh samples. Here we present a simple method to assess the activity of mitochondria respiratory complexes I and II in previously cryopreserved murine skeletal muscle tissue homogenates, as well as previously frozen ''D. melanogaster'', as a function of oxygen consumption. | ||
|editor=[[Plangger M]] | |editor=[[Plangger M]] | ||
|mipnetlab=UK Nottingham Chakrabarti L, UK Leicester Moisoi N | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration, Instruments;methods | ||
|injuries=Cryopreservation | |||
|organism=Mouse, Drosophila | |||
|tissues=Skeletal muscle | |||
|preparations=Homogenate | |||
|couplingstates=LEAK, ROUTINE | |||
|pathways=N, S, NS, ROX | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=2023-07 | |additional=2023-07 | ||
}} | }} | ||
Latest revision as of 17:56, 31 July 2023
| Ebanks B, Kwiecinska P, Moisoi N, Chakrabarti L (2023) A method to assess the mitochondrial respiratory capacity of complexes I and II from frozen tissue using the Oroboros O2k-FluoRespirometer. https://doi.org/10.1371/journal.pone.0276147 |
» PLoS One 18:e0276147. PMID: 37486925 Open Access
Ebanks Brad, Kwiecinska Pola, Moisoi Nicoleta, Chakrabarti Lisa (2023) PLoS One
Abstract: High-resolution respirometry methods allow for the assessment of oxygen consumption by the electron transfer systems within cells, tissue samples, and isolated mitochondrial preparations. As mitochondrial integrity is compromised by the process of cryopreservation, these methods have been limited to fresh samples. Here we present a simple method to assess the activity of mitochondria respiratory complexes I and II in previously cryopreserved murine skeletal muscle tissue homogenates, as well as previously frozen D. melanogaster, as a function of oxygen consumption.
• Bioblast editor: Plangger M • O2k-Network Lab: UK Nottingham Chakrabarti L, UK Leicester Moisoi N
Labels: MiParea: Respiration, Instruments;methods
Stress:Cryopreservation Organism: Mouse, Drosophila Tissue;cell: Skeletal muscle Preparation: Homogenate
Coupling state: LEAK, ROUTINE
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k
2023-07
