MiPNet06.06 Chemical O2 background
Kuznetsov AV, Gnaiger E (2001) Oxygraph assay of cytochrome c oxidase activity: Chemical background correction. Mitochondr Physiol Network 06.06. |
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OROBOROS (2001) Mitochondr Physiol Network
Abstract: [[has abstract::O2k-Protocol for chemical background correction
Oxygraphic determination of cytochrome c oxidase activity in the presence of TMPD, ascorbate and cytochrome c requires consideration of chemical background oxygen consumption. Several compounds are readily oxidized by molecular oxygen when dissolved in water. This leads to a significant chemical oxygen consumption in the absence of any respiring biological sample. The rate of this autoxidation represents a chemical background which strongly depends on experimental conditions, such as temperature, chemical composition, pH and oxygen concentration. Moreover, the rate of autoxidation may be significantly catalyzed by metal traces and metal-containing proteins (e.g. cytochrome c). Therefore, high-resolution respirometric measurement of oxygen flux ultimately depends on chemical background correction analyzed under experimental conditions over the entire oxygen range.
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue]]
β’ O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
Preparation: Enzyme, Oxidase; Biochemical Oxidation
HRR: Oxygraph-2k, Protocol
O2k-SOP
Further information
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- Version 7: 2010-08-23
- Version 1: 2001