high-resolution terminology - matching measurements at high-resolution

OXPHOS capacity

Description

[[Description:: OXPHOS capacity (P) is the respiratory capacity of mitochondria in the ADP-activated state of oxidative phosphorylation, at saturating concentrations of ADP (possibly in contrast to State 3), inorganic phosphate, oxygen, and defined reduced substrates. » MiPNet article]]

Abbreviation: Has abbr::''P''

Reference: [[Info::Gnaiger 2014 MitoPathways, Gnaiger 2009 Int J Biochem Cell Biol]]

MitoPedia concepts: Respiratory state, SUIT concept, Recommended

MitoPedia methods: Respirometry

MitoPedia topics: MitoPedia topic::EAGLE

» Keywords

» ADP

» CS

» Dyscoupling

» Flow

» Flux control ratio

» Free OXPHOS capacity

» Gnaiger 2014 MitoPathways

» Oxidative phosphorylation

» Oxygen flow

» Oxygen flux

» P/E

» State 3

» Uncoupling

Coupled energy cycles of oxidative phosphorylation stimulated by saturating concentrations of ADP and inorganic phosphate. 2[H] indicates the reduced hydrogen equivalents of CHO substrates (NS) and electron transfer to oxygen. H⁺_out are protons pumped out of the matrix phase to the positive P-phase. Proton flow to the negative matrix phase (H⁺_in) drives the phosphorylation of ADP to ATP. The capacity of the phosphorylation system may contribute to the limitation of flux. Proton leaks dissipate energy of translocated protons from the positive P-phase to the negative N-phase. Measurement of OXPHOS capacity is possible in mt-preparations supported by an ETS-competent substrate state, exemplifed as the NS-pathway (CI&II-linked substrate supply) (Gnaiger 2014 MitoPathways).

OXPHOS (P) and State 3

Has title::Gnaiger E (2014) OXPHOS (''P'') and State 3. Mitochondr Physiol Network 2016-07-15.

Was written by::OROBOROS (Was published in year::2016) Was published in journal::MiPNet

Abstract: [[has abstract:: OXPHOS capacity (P) is the respiratory capacity of mitochondria in the ADP-activated state of oxidative phosphorylation, at saturating concentrations of ADP (possibly in contrast to State 3), inorganic phosphate, oxygen, and defined reduced substrates. Since OXPHOS is partially coupled, intrinsic uncoupling and dyscoupling contribute to the control of flux in the OXPHOS state (State P). Oxygen consumption in the OXPHOS state, P, therefore, is partitioned into the free OXPHOS capacity, ≈P, strictly coupled to phosphorylation, ~P, and nonphosphorylating LEAK respiration, L_P, compensating for proton leaks, slip and cation cycling: P = ≈P+L_P. It is frequently assumed that LEAK respiration, L, as measured in the LEAK state, overestimates the LEAK component of respiration, L_P, as measured in the OXPHOS state, particularly if the protonmotive force is not adjusted to equivalent levels in L and L_P. However, if the LEAK component increases with enzyme turnover during P, the low enzyme turnover during L may counteract the effect of the higher Δp_mt. OXPHOS capacity is expressed (i) per mt-marker (O₂ flux per mt-protein, CS, etc); if ETS capacity, E, is used as a functional mitochondrial marker, then OXPHOS capacity is expressed as the P/E ratio (flux control ratio). (ii) OXPHOS capacity is expressed per tissue or cell mass, integrating mt-quantity (density) and mt-quality (O₂ flux). (iii) OXPHOS capacity is expressed per cell (O₂ flow), which then is a function of mt-density, mt-quality, and cell size. If conditions for measurement and expression of respiration vary, explicit symbols are used, expressing OXPHOS capacity as oxygen flux in state P, J_O2P or as oxygen flow in state P, I_O2P. If these conditions are defined and remain consistent within a given context, then the simple symbol P for respiratory state can be used to substitute the more explicit expression for respiratory activity.]]

• O2k-Network Lab: Was published by MiPNetLab::AT Innsbruck Gnaiger E

Labels:

Coupling state: Coupling states::OXPHOS

HRR: Instrument and method::Theory

Related terms in Bioblast

OXPHOS, P

ROUTINE, R

ETS, E

LEAK, L

ROX, R

Free OXPHOS capacity, ≈P = P-L
Free ROUTINE activity, ≈R = R-L
Free ETS capacity, ≈E = E-L
Excess E-P capacity, ExP = E-P
Excess E-R capacity, ExR = E-R

» List of publications: OXPHOS

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{{#ask: Coupling states::OXPHOS |?Was published in year=Year |?Has title=Reference |?Coupling states=Coupling |?Pathways=Pathway |?Preparation=Preparation |?Mammal and model=Organism |?Tissue and cell=Tissue;cell |format=broadtable |limit=5000 |offset=0 |sort=Was published in year |order=descending }}

Abstracts: OXPHOS

Sort in ascending/descending order by a click on one of the small symbols in squares below.

{{#ask: Coupling states::OXPHOS |?Was submitted in year=Year |?Has title=Reference |?Coupling states=Coupling |?Pathways=Pathway |?Preparation=Preparation |?Mammal and model=Organism |?Tissue and cell=Tissue;cell |format=broadtable |limit=5000 |offset=0 |sort=Was submitted in year |order=descending }}