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Calzia 2012 Abstract Bioblast

From Bioblast
Matallo J, Groeger M, Wachter U, Vogt J, Radermacher P, Calzia E (2012) Interactions between sulfide metabolism and oxygen concentration. Mitochondr Physiol Network 17.12.

Link: MiPNet17.12 Bioblast 2012 - Open Access

Matallo J, Groeger M, Wachter U, Vogt J, Radermacher P, Calzia E (2012)

Event: Bioblast 2012

Enrico Calzia

Even in higher species sulfide is quickly metabolised and thus degraded by an ancient metabolic pathway linked to the mitochondrial respiratory chain [1]. Since this metabolic process is strictly aerobic, the efficiency of sulfide metabolism is expected to decrease at low oxygen. This mechanism may explain how sulfide acts as an oxygen sensor within cells [2]. Therefore, in a series of preliminary experiments we quantified the sulfide metabolism in a stable cell line derived from alveolar macrophages (AMJ2-C11), which previously proved to efficiently metabolise sulfide under aerobic conditions [3], at O2 partial pressures approaching hypoxia from 0.8 kPa down to 0.4 and 0.1 kPa.

Methods: All measurements were conducted using an Oxygraph-2k together with a TIP2k® titration pump (Oroboros Instruments, Austria). For each O2-partial pressure we performed 6 – 8 separate experiments. Before performing the experiments we recorded several transitions to anoxia in order to quantify the un-inhibited respiratory capacity close to anoxia [4] under the O2-partial pressures of 0.1, 0.4, and 0.8 KPa respectively. The inhibition of mitochondrial respiration was quantified in terms of the total amount of sulfide required to reduce the routine oxygen flux (JO2) to 50% by means of a continuous sulfide injection at a rate of 10 nM/s. A second titration pump was used to simultaneously maintain the oxygen concentration rate at the predefined level adopting a previously described technique [5].

Results: Figure 1 shows representative records of 2 anoxic transition experiments in AMJ2-C11 cells; the JO2 was 74%, 92%, and 96% of maximum JO2 [JO2max] at 0.1 kPa, 0.4 kPa, and 0.8 kPa. The total amount of sulfide required for achieving a 50%-inhibition of mitochondrilal respiration was about 10% at 0.1 kPa when compared to that needed at 0.8 kPa. (0.2 ± 0.03 µmol at 0.1 kPa vs. 1.30 ± 0.4 µmol at 0.4 kPa, and 2.2 ± 1.1 µmol at 0.8 kPa; see Figure 2).

Discussion: These results confirm a much less efficient degradation of sulfide at the lower oxygen level suggesting potential interactions between sulfide and cell metabolism when approaching the anoxic condition. In theory, sulfide concentration should increase and thus potentially inhibit mitochondrial respiration at low oxygen, provided that the production rate is sufficiently high and remains constant under these conditions, and that the excess sulfide is not chemically bound by proteins (sulfhydration). On the other hand, the inhibition of mitochondrial respiration should in turn decrease the oxygen consumption of the cell, thus potentially decelerating or even impeding a further decrease of the oxygen concentration. So far, however, whether and how sulfide interacts with metabolism at low oxygen remains the subject of future investigations.

  1. Bouillaud F, Blachier F (2011) Mitochondria and sulfide: a very old story of poisoning, feeding, and signaling? Antioxid Redox Signal 15: 379-391.
  2. Olson KR (2012) Mitochondrial adaptations to utilize hydrogen sulfide for energy and signaling. J Comp Physiol B 182: 881-897.
  3. Groeger M, Matallo J, McCook O, Wagner F, Wachter U, Bastian O, Gierer S, Reich V, Stahl B, Huber-Lang M, Szabó C, Georgieff M, Radermacher P, Calzia E, Wagner K (2012) Temperature and cell-type dependency of sulfide effects on mitochondrial respiration. Shock 38: 367-374.

Keywords: Hydrogen sulfide, Metabolic pathways

O2k-Network Lab: DE Ulm Radermacher P

Labels: MiParea: Respiration 

Organism: Mouse  Tissue;cell: Macrophage-derived  Preparation: Intact cells 

Regulation: Aerobic glycolysis, Inhibitor, Oxygen kinetics 

HRR: Oxygraph-2k, TIP2k 

Affiliations and author contributions

Division APV, Dept. Anesthesiology, University Hospital, Ulm, Germany; Email: [email protected]

Supplementary references

4. Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply Respir Physiol 128: 277-297.

5. Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics Adv Exp Med Biol 662: 7-25.

Figure 1

Representative records of 2 anoxic transition experiments in AMJ2-C11 cells

The figure shows original data on JO2/JO2max from two representative anoxic transition experiments conducted with AMJ2-C11 cells (open dots). As indicated by the vertical arrows, the sulfide titrations have been performed under O2-partial pressures of 0.1, 0.4, and 0.8 kPa, corresponding to a JO2 of 74%, 92%, and 96% of JO2max respectively.

Figure 2

Injected amount of sulfide

The figure plots the injected amount of sulfide against the JO2 at all three partial pressures.