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Ciminale 2016 Abstract Mito Xmas Meeting Innsbruck

From Bioblast
p13, a mitochondrial protein coded by human T-cell leukemia virus type 1.

Link:

Ciminale V, Biasiotto R, Ilaria Cavallari, Silic-Benussi M, D’Agostino DM (2016)

Event: Mito Xmas Meeting 2016 Innsbruck AT

Human T-cell leukemia virus type 1 (HTLV-1) is a complex retrovirus that causes a T-cell neoplasm termed adult T-cell leukemia/lymphoma and a neurodegenerative disease termed tropical spastic paraparesis/HTLV-associated myelopathy. HTLV-1 expresses several regulatory and accessory proteins, including p13, an 87-amino acid protein that is targeted to the inner mitochondrial membrane through the action of an arginine- and leucine-rich mitochondrial targeting signal (MTS). In mitochondria, p13 opens a K+ selective channel, resulting in an inward K+ current that enhances the activity of the respiratory chain, increases production of ROS and leads to membrane depolarization and mitochondrial fragmentation. These effects are linked to the protein’s impact on cell turnover, which include activation of primary T-cells and reduced proliferation and sensitization to death signals in transformed cells. Further investigation of the factors influencing the intracellular localization and activity of p13 showed that it accumulates both in mitochondria and in the nucleus when expressed at high levels. Results of mutational analyses indicated that a serine residue located near the MTS modulates p13’s activities, as its substitution with alanine abrogated mitochondrial fragmentation and loss of membrane potential, while substitution with aspartic acid led to pronounced fragmentation and collapse of membrane potential and partial localization of the protein to the cytosol and nucleus. Current studies are aimed identifying possible posttranslational modifications on the serine residue that might play a role in controlling p13’s function.


Labels: MiParea: mt-Medicine  Pathology: Cancer, Infectious  Stress:Oxidative stress;RONS  Organism: Human  Tissue;cell: Lymphocyte  Preparation: Intact cells, Isolated mitochondria 

Regulation: Ion;substrate transport, mt-Membrane potential, Redox state 


Event: Poster  Labelled by author 

Affiliations

Ciminale V(1,2), Biasiotto R(1), Ilaria Cavallari(1), Silic-Benussi M(2), D’Agostino DM(3)


  1. Dept Surgery, Oncology and Gastroenterology, Univ Padova, Italy
  2. Ist Oncologico Veneto-IRCCS, Padova, Italy
  3. Dept Biomedical Sc, Univ Padova, Italy