Fisar 2023 Biomedicines

Fišar Z, Hroudová J, Zvěřová M, Jirák R, Raboch J, Kitzlerová E (2023) Age-dependent alterations in platelet mitochondrial respiration. https://doi.org/10.3390/biomedicines11061564

» Biomedicines 11:1564. PMID: 37371659 Open Access

Fisar Zdenek, Hroudova Jana, Zverova Martina, Jirak Roman, Raboch Jiri, Kitzlerova Eva (2023) Biomedicines

Abstract: Mitochondrial dysfunction is an important cellular hallmark of aging and neurodegeneration. Platelets are a useful model to study the systemic manifestations of mitochondrial dysfunction. To evaluate the age dependence of mitochondrial parameters, citrate synthase activity, respiratory chain complex activity, and oxygen consumption kinetics were assessed. The effect of cognitive impairment was examined by comparing the age dependence of mitochondrial parameters in healthy individuals and those with neuropsychiatric disease. The study found a significant negative slope of age-dependence for both the activity of individual mitochondrial enzymes (citrate synthase and complex II) and parameters of mitochondrial respiration in intact platelets (routine respiration, maximum capacity of electron transport system, and respiratory rate after complex I inhibition). However, there was no significant difference in the age-related changes of mitochondrial parameters between individuals with and without cognitive impairment. These findings highlight the potential of measuring mitochondrial respiration in intact platelets as a means to assess age-related mitochondrial dysfunction. The results indicate that drugs and interventions targeting mitochondrial respiration may have the potential to slow down or eliminate certain aging and neurodegenerative processes. Mitochondrial respiration in platelets holds promise as a biomarker of aging, irrespective of the degree of cognitive impairment. • Keywords: Aging, Cognitive decline, Mitochondria, Mitochondrial respiration, Neurodegenerative disease, Neuroinflammation, Neuroplasticity, Oxidative stress, Platelet, Respiratory chain complex • Bioblast editor: Plangger M • O2k-Network Lab: CZ Prague Fisar Z

Labels: MiParea: Respiration, Patients  Pathology: Aging;senescence, Neurodegenerative 

Organism: Human  Tissue;cell: Platelet  Preparation: Intact cells 

Coupling state: LEAK, ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k 

2023-06