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Flux control ratio |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
Flux control ratios FCR are ratios of oxygen flux in different respiratory control states, normalized for maximum flux in a common reference state, to obtain theoretical lower and upper limits of 0.0 and 1.0 (0 % and 100 %).
For a given protocol or set of respiratory protocols, flux control ratios provide a fingerprint of coupling and substrate control independent of (1) mt-content in cells or tissues, (2) purification in preparations of isolated mitochondria, and (3) assay conditions for determination of tissue mass or mt-markers external to a respiratory protocol (CS, protein, stereology, etc.). FCR obtained from a single respirometric incubation with sequential titrations (sequential protocol; SUIT protocol) provide an internal normalization, expressing respiratory control independent of mitochondrial content and thus independent of a marker for mitochondrial amount. FCR obtained from separate (parallel) protocols depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common mitochondrial marker.
Abbreviation: FCR
Reference: Gnaiger 2020 BEC MitoPathways, Gnaiger 2009 Int J Biochem Cell Biol, Doerrier 2018 Methods Mol Biol
Flux control efficiency: normalization of mitochondrial respiration
- ยป More details: Flux control efficiency
DatLab
Unknown sample concentration and normalization per unit sample [x]
- In the DatLab 7.4 Excel template for oxygen flux analysis (O2 analysis template DL7.4):
- If the sample concentration is not yet known, the box โKnown sample concentrationโ can be unchecked, and the concentration will be considered by default as 1, with units [xยทmL-1]. In this way, flux can be normalized and FCRs can be obtained even if the sample concentration is unknown.
- ยป Read also: Extensive quantity; BEC 2020.1
- ยป More details: MiPNet24.06 Oxygen flux analysis - DatLab 7.4
FCR in DatLab plot
- The entire oxygen flux plot can be converted to a FCR. Click on 'Flux/Slope' in the DatLab pull-down menu. Select chamber A or B 'O2 slope'. Select 'Flux control ratio, FCR' and select the mark that corresponds to the reference state. Change the layout under scale under 'Layout/Standard layouts' and select '07a Flux Control Ratios' or '07b Flux Control Ratios overlay'.
- ยป More details: Flux control ratio
References
Bioblast link | Reference | Year |
---|---|---|
Doerrier 2018 Methods Mol Biol | Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mรฉszรกros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. https://doi.org/10.1007/978-1-4939-7831-1_3 | 2018 |
Gnaiger 2009 Int J Biochem Cell Biol | Gnaiger E (2009) Capacity of oxidative phosphorylation in human skeletal muscle. New perspectives of mitochondrial physiology. https://doi.org/10.1016/j.biocel.2009.03.013 | 2009 |
Gnaiger 2020 BEC MitoPathways | Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. https://doi.org/10.26124/bec:2020-0002 | 2020 |
BEC 2020.1 doi10.26124bec2020-0001.v1 | Gnaiger E et al โ MitoEAGLE Task Group (2020) Mitochondrial physiology. https://doi.org/10.26124/bec:2020-0001.v1 | 2020 |
Keywords
4-compartmental OXPHOS model. (1) ET capacity E of the noncoupled electron transfer system ETS. OXPHOS capacity P is partitioned into (2) the dissipative LEAK component L, and (3) ADP-stimulated P-L net OXPHOS capacity. (4) If P-L is kinetically limited by a low capacity of the phosphorylation system to utilize the protonmotive force pmF, then the apparent E-P excess capacity is available to drive coupled processes other than phosphorylation Pยป (ADP to ATP) without competing with Pยป.
- Bioblast links: Coupling control - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
1. Mitochondrial and cellular respiratory rates in coupling-control states
Respiratory rate | Defining relations | Icon | |
---|---|---|---|
OXPHOS capacity | P = Pยด-Rox | ![]() |
mt-preparations |
ROUTINE respiration | R = Rยด-Rox | ![]() |
living cells |
ET capacity | E = Eยด-Rox | ![]() |
ยป Level flow |
ยป Noncoupled respiration - Uncoupler | |||
LEAK respiration | L = Lยด-Rox | ![]() |
ยป Static head |
ยป LEAK state with ATP | |||
ยป LEAK state with oligomycin | |||
ยป LEAK state without adenylates | |||
Residual oxygen consumption Rox | L = Lยด-Rox | ![]() |
2. Flux control ratios related to coupling in mt-preparations and living cells
FCR | Definition | Icon | |
---|---|---|---|
L/P coupling-control ratio | L/P | ![]() |
ยป Respiratory acceptor control ratio, RCR = P/L |
L/R coupling-control ratio | L/R | ![]() | |
L/E coupling-control ratio | L/E | ![]() |
ยป Uncoupling-control ratio, UCR = E/L (ambiguous) |
P/E control ratio | P/E | ![]() | |
R/E control ratio | R/E | ![]() |
ยป Uncoupling-control ratio, UCR = E/L |
net P/E control ratio | (P-L)/E | ![]() | |
net R/E control ratio | (R-L)/E | ![]() |
3. Net, excess, and reserve capacities of respiration
Respiratory net rate | Definition | Icon |
---|---|---|
P-L net OXPHOS capacity | P-L | ![]() |
R-L net ROUTINE capacity | R-L | ![]() |
E-L net ET capacity | E-L | ![]() |
E-P excess capacity | E-P | ![]() |
E-R reserve capacity | E-R | ![]() |
4. Flux control efficiencies related to coupling-control ratios
- ยป Flux control efficiency jZ-Y
- ยป Background state
- ยป Reference state
- ยป Metabolic control variable
Coupling-control efficiency | Definition | Icon | Canonical term | ||
---|---|---|---|---|---|
P-L control efficiency | jP-L | = (P-L)/P | = 1-L/P | ![]() |
P-L OXPHOS-flux control efficiency |
R-L control efficiency | jR-L | = (R-L)/R | = 1-L/R | ![]() |
R-L ROUTINE-flux control efficiency |
E-L coupling efficiency | jE-L | = (E-L)/E | = 1-L/E | ![]() |
E-L ET-coupling efficiency ยป Biochemical coupling efficiency |
E-P control efficiency | jE-P | = (E-P)/E | = 1-P/E | ![]() |
E-P ET-excess flux control efficiency |
E-R control efficiency | jE-R | = (E-R)/E | = 1-R/E | ![]() |
E-R ET-reserve flux control efficiency |
5. General
- ยป Basal respiration
- ยป Cell ergometry
- ยป Dyscoupled respiration
- ยป Dyscoupling
- ยป Electron leak
- ยป Electron-transfer-pathway state
- ยป Hyphenation
- ยป Oxidative phosphorylation
- ยป Oxygen flow
- ยป Oxygen flux
- ยป Permeabilized cells
- ยป Phosphorylation system
- ยป Proton leak
- ยป Proton slip
- ยป Respiratory state
- ยป Uncoupling
- Bioblast links: Normalization - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Rate
- ยป Normalization of rate
- ยป Flow
- ยป Oxygen flow
- ยป Flux
- ยป Oxygen flux
- ยป Flux control ratio
- ยป Coupling-control ratio
- ยป Pathway control ratio
- ยป Flux control efficiency
- Rate
- Quantities for normalization
- ยป Count in contrast to Number
- ยป Mitochondrial marker
- ยป O2k-Protocols: mitochondrial and marker-enzymes
- ยป Citrate synthase activity
- Quantities for normalization
- General
- ยป Extensive quantity
- ยป Specific quantity
- ยป Advancement
- ยป Motive unit
- ยป Iconic symbols
- General
- Related keyword lists
MitoPedia concepts:
Respiratory control ratio,
SUIT concept
MitoPedia methods:
Respirometry
MitoPedia O2k and high-resolution respirometry:
DatLab