Sivitz 2017 Neuromethods

Sivitz WI (2017) Techniques to investigate bioenergetics of mitochondria. In: Strack S, Usachev Y (eds) Techniques to investigate mitochondrial function in neurons. Neuromethods 123:67-94. Humana Press, New York. https://doi.org/10.1007/978-1-4939-6890-9_4

» Springer Science

Sivitz WI (2017) Neuromethods

Abstract: In this chapter we review basic mitochondrial physiology as related to respiration, membrane potential, and ATP production and the relationship to ROS and calcium . Methods for measuring various functional parameters are described. Techniques differ according to whether one is assessing isolated mitochondria or intact cells. Although isolated mitochondria remain the only way to evaluate properties intrinsic to the organelles per se, mitochondrial function clearly is dependent on a multitude of cellular and whole body factors.

• Bioblast editor: Gnaiger E • O2k-Network Lab: US IA Iowa City Sivitz WI

Correction: FADH₂ and Complex II

FADH₂ is shown as the substrate feeding electrons into Complex II (CII). This is wrong and requires correction - for details see Gnaiger (2024).

Gnaiger E (2024) Complex II ambiguities ― FADH₂ in the electron transfer system. J Biol Chem 300:105470. https://doi.org/10.1016/j.jbc.2023.105470 - »Bioblast link«

Labels: MiParea: Respiration 

Preparation: Isolated mitochondria, Intact cells 

Coupling state: LEAK, OXPHOS, ET  Pathway: F, N, S  HRR: Oxygraph-2k