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  • ...AV, Brandacher G, Steurer W, Margreiter R, Gnaiger E (1999) Estimation of mitochondrial damage in heart preservation. Transplant Proc 31:992. ...spirometry with permeabilized cells and muscle fibers for investigation of mitochondrial cold ischemia/reperfusion (CIR) injury.
    1 KB (120 words) - 04:24, 23 November 2021
  • ...content decreases respiratory chain capacities and increases ATP synthesis yield in the human HepaRG cells. Biochim Biophys Acta 1857:443-53. ...entation. In addition, CL content may regulate mitochondrial ATP synthesis yield.
    3 KB (392 words) - 23:54, 18 March 2018
  • |title=Cardoso LHD, Gnaiger E (2022) Reverse citric acid cycle and mitochondrial succinate formation under anoxia: from parasites and invertebrates to ische ...t profound implications on the redox balance (Harrison et al 2015) and ATP yield (Gnaiger 1991).
    2 KB (349 words) - 10:00, 26 February 2022
  • ...paration technique depends on the requirements regarding organelle purity, yield, activity and also the duration of the preparation. The most widely used me ...production (DCFDA) and mitochondrial membrane potential (Safranine O). The yield, purity and the loss of mitochondria were monitored by measuring enzyme act
    4 KB (548 words) - 10:56, 18 March 2020
  • ...lly, compared with the PCV2 yield of infected control PK15 cells, the PCV2 yield of infected Aralar I over-expression cells was enhanced by 42.5% (p < 0.05)
    2 KB (279 words) - 13:21, 25 February 2020
  • ...Donhauser E, Maske T, Bischof C, Dumitrescu D, Rudolph V, Klinke A (2023) Mitochondrial integrity is critical in right heart failure development. Int J Mol Sci 24: ...clinical and clinical RVD and RHF and to decipher the relationship between mitochondrial processes and the functional aspects of the right ventricle (RV).
    2 KB (220 words) - 17:48, 13 October 2023
  • ...sing RT-qPCR, the sarcomere organization using immunofluorescence, and the mitochondrial function using oxidative respiration analysis. ...g contractile- and ion transport-associated gene expression, and enhancing mitochondrial respiration. M0 macrophages did not significantly affect the cardiomyogenes
    3 KB (390 words) - 14:49, 31 August 2022
  • ...10%-15%. Mitochondrial experiments invoke a trio of titration protocols to yield information on oxygen consumption, ATP flux, membrane potential, electron l ...t production, Mitochondrial membrane potential, Mitochondrial proton leak, Mitochondrial reactive oxygen species production
    2 KB (222 words) - 11:40, 9 February 2022
  • ...Kummitha CM, Rosca MG, Fujioka H, Tandler B, Hoppel CL (2019) Isolation of mitochondrial subpopulations from skeletal muscle: Optimizing recovery and preserving int ...ehensive approach is developed to identify the best conditions to separate mitochondrial fractions.
    2 KB (317 words) - 08:44, 16 July 2021
  • Changes of exergy d''G'' plus [[bound energy]] yield the [[enthalpy]] change: :::# Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th e
    1 KB (134 words) - 05:57, 16 September 2022
  • |title=Werner C, Natter R, Schellander K, Wicke M (2010) Mitochondrial respiratory activity in porcine longissimus muscle fibers of different pig ...larger than those of the other genetics (''P''<0.05). The analysis of the mitochondrial respiratory activity (MRA) using permeabilized longissimus muscle fibers re
    2 KB (244 words) - 10:51, 27 March 2018
  • |title=Divakaruni AS, Rogers GW, Murphy AN (2014) Measuring mitochondrial function in permeabilized cells using the Seahorse XF Analyzer or a clark-t ...tic understanding. Mitochondria can be isolated from intact cells, but the yield and quality of the end product is often poor and prone to subselection duri
    2 KB (229 words) - 17:46, 9 November 2017
  • ...Kummitha C, Rosca MG, Fujioka H, Tandler B, Hoppel CL (2018) Isolation of mitochondrial subpopulations from skeletal muscle: optimizing recovery and preserving int ...ehensive approach is developed to identify the best conditions to separate mitochondrial fractions.
    3 KB (362 words) - 17:49, 25 January 2021
  • ...the largest enzyme of respiratory chain and the most commonly affected in mitochondrial diseases. Mammalian CI consists of 44 different subunits organized in a mod ...n dysfunction and identifies CLPP as an exciting new target for therapy of mitochondrial diseases.
    2 KB (315 words) - 14:55, 8 October 2019
  • ...relative to mitochondrial protein. The pig tissue contains ten times less mitochondrial protein than the pigeon tissue does.
    2 KB (309 words) - 01:21, 16 February 2022
  • |title=Taroni F, Gellera C, Di Donato S (1987) Evidence for two distinct mitochondrial malic enzymes in human skeletal muscle: Purification and properties of the ...raphy. The NAD+/NADP+-dependent enzyme was purified 1600-fold with a final yield of 34% and a final specific activity of 32.9 units/mg of protein by employi
    2 KB (272 words) - 15:10, 27 May 2020
  • ...ed, islet isolation still suffers from large variability in terms of islet yield and quality. A comprehensive quality control for isolated and purified isle ...clinical setting. We hypothesize, that analysis of membrane integrity and mitochondrial function by high-resolution respirometry may provide a guideline for furthe
    2 KB (282 words) - 04:24, 23 November 2021
  • ...4) Polarographic studies of saponin-skinned muscle fibers in patients with mitochondrial myopathies. J Inher Metab Dis 17:307-10. ...''., 1987) and human muscle tissue. In such permeabilized fibers, study of mitochondrial respiratory control is possible as in isolated mitochondria but without the
    2 KB (331 words) - 04:39, 23 November 2021
  • ...in high-altitude deer mice. Therefore, physiological variation in specific mitochondrial fractions can help overcome the metabolic challenges of life at high altitu
    3 KB (340 words) - 17:44, 23 November 2022
  • ...hend MR, Tanakac H, Iida-Tanakab N (2015) Knockdown of aquaporin-8 induces mitochondrial dysfunction in 3T3-L1 cells. Biochem Biophys Rep 4:187–95. ...nctions on the plasma membrane and/or on the mitochondrial inner membrane. Mitochondrial AQP8 with permeability for water, H<sub>2</sub>O<sub>2</sub> and NH<sub>3</
    3 KB (390 words) - 14:42, 13 November 2017

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