Lhuissier 2024 iScience: Difference between revisions

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{{Labeling
{{Labeling
|area=Respiration, mt-Structure;fission;fusion
|area=Respiration, mt-Structure;fission;fusion
|instruments=Oxygraph-2k
|topics=mt-Membrane potential
|instruments=Oxygraph-2k, O2k-Fluorometer
|additional=2024-05
|additional=2024-05
}}
}}

Revision as of 14:43, 14 May 2024

Publications in the MiPMap
Lhuissier C, Desquiret-Dumas V, Girona A, Alban J, Faure J, Cassereau J, Codron P, Lenaers G, Baris OR, Gueguen N, Chevrollier A (2024) Mitochondrial F0F1-ATP synthase governs the induction of mitochondrial fission. iScience 27:109808. https://doi.org/10.1016/j.isci.2024.109808

ยป PMID: 38741710 Open Access

Lhuissier Charlene, Desquiret-Dumas Valerie, Girona Anais, Alban Jennifer, Faure Justine, Cassereau Julien, Codron Philippe, Lenaers Guy, Baris Olivier R, Gueguen Naig, Chevrollier Arnaud (2024) iScience

Abstract: Mitochondrial dynamics is a process that balances fusion and fission events, the latter providing a mechanism for segregating dysfunctional mitochondria. Fission is controlled by the mitochondrial membrane potential (ฮ”ฮจm), optic atrophy 1 (OPA1) cleavage, and DRP1 recruitment. It is thought that this process is closely linked to the activity of the mitochondrial respiratory chain (MRC). However, we report here that MRC inhibition does not decrease ฮ”ฮจm nor increase fission, as evidenced by hyperconnected mitochondria. Conversely, blocking F0F1-ATP synthase activity induces fragmentation. We show that the F0F1-ATP synthase is sensing the inhibition of MRC activity by immediately promoting its reverse mode of action to hydrolyze matrix ATP and restoring ฮ”ฮจm, thus preventing fission. While this reverse mode is expected to be inhibited by the ATPase inhibitor ATPIF1, we show that this sensing is independent of this factor. We have unraveled an unexpected role of F0F1-ATP synthase in controlling the induction of fission by sensing and maintaining ฮ”ฮจm. โ€ข Keywords: Biochemistry, Cell biology, Functional aspects of cell biology โ€ข Bioblast editor: Plangger M โ€ข O2k-Network Lab: FR Angers Gueguen N


Labels: MiParea: Respiration, mt-Structure;fission;fusion 




Regulation: mt-Membrane potential 


HRR: Oxygraph-2k, O2k-Fluorometer 

2024-05 

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