SUIT protocol library: Difference between revisions
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== List of SUIT numbers == | == List of SUIT numbers == | ||
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== List of SUIT protocols with D-numbers == | == List of SUIT protocols with D-numbers == | ||
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| ?Has SUIT number=SUIT technical name | | ?Has SUIT number=SUIT technical name | ||
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Revision as of 16:03, 15 January 2019
Description
The Substrate-uncoupler-inhibitor titration (SUIT) protocol library contains a sequential list of SUIT protocols (D01, D02, ..) with links to the specific SUIT pages. Classes of SUIT protocols are explained with coupling and substrate control defined for mitochondrial preparations.
Abbreviation: SUITs
Reference: MitoPedia: SUIT
List of SUIT numbers
List of SUIT protocols with D-numbers
SUIT code | SUIT technical name |
---|---|
SUIT-001 O2 ce-pce D003 | D003_ce1;1Dig;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd |
SUIT-001 O2 ce-pce D004 | D004_ce1;1Dig;1PM;2D;2c;3U;4G;5S;(6Oct);7Rot;8Gp;9Ama;10AsTm;11Azd |
SUIT-001 O2 mt D001 | D001_mt;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd |
SUIT-001 O2 pfi D002 | D002_pfi;1PM;2D;2c;3U;4G;5S;6Oct;7Rot;8Gp;9Ama;10AsTm;11Azd |
SUIT-002 O2 ce-pce D007 | D007_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd |
SUIT-002 O2 ce-pce D007a | D007a_ce1;1Dig;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd |
SUIT-002 O2 mt D005 | D005_mt;1D;2M;3Oct;3c;4M;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd |
SUIT-002 O2 pfi D006 | D006_pfi;1D;2M.1;3Oct;3c;4M2;5P;6G;7S;8Gp;9U;10Rot;11Ama;12AsTm;13Azd |
SUIT-003 AmR ce D017 | D017_ce1;ce2P;ce3Omy;ce4U;ce5Rot;ce6S;ce7Ama |
SUIT-003 AmR ce D058 | D058_ce1;ce1SOD;ce1HRP;ce1AmR;ce2Omy;ce3U;ce4Rot;ce5Ama |
SUIT-003 AmR ce D059 | D059_ce1;ce1H2O;ce1MiR05;ce1DMSO;ce2Omy;ce3U;ce4Rot;ce5Ama |
SUIT-003 O2 ce D009 | D009_ce1;ce2Omy;ce3U;ce4Ama |
SUIT-003 O2 ce D012 | D012_ce1;ce1P;ce2Omy;ce3U;ce4Rot;ce5Ama |
SUIT-003 O2 ce D028 | D028_ce1;ce2Omy;ce3U;ce4Rot;ce5S;ce6Ama |
SUIT-003 O2 ce D037 | D037_ce1;ce1Glc;(ce2Omy);ce3U;ce4Ama |
SUIT-003 O2 ce D038 | D038_ce1;(ce2Omy);ce3U;ce3Glc;ce3'U;ce4Ama |
SUIT-003 O2 ce D039 | D039_ce1;(ce2Omy);ce3U;ce4Rot;ce5Ama |
SUIT-003 O2 ce D050 | D050_ce1;ce1Snv;(ce2Omy);ce3U;ce4Rot;ce5Ama |
SUIT-003 O2 ce D060 | D060_ce1;(ce2Omy);ce3U;ce4Rot;ce5Snv;ce6Mnanv;ce7Ama |
SUIT-003 O2 ce D061 | D061_ce1;(ce2Omy);ce3U;ce4Rot;ce5DMSO;ce6DMSO;ce7Ama |
SUIT-003 O2 ce D062 | D062_ce1;ce1DMSO;(ce2Omy);ce3U;ce4Rot;ce5Ama |
SUIT-003 O2 ce-pce D013 | D013_ce1;ce1P;ce2Omy;ce3U;ce3Glc;ce3M;ce4Rot;ce5S;1Dig;1U;1c;2Ama;3AsTm;4Azd |
SUIT-003 O2 ce-pce D018 | D018_ce1;ce1P;ce2Omy;ce3U;ce3Glc;ce4Rot;ce5S;1Dig;1c;2Ama;3AsTm;4Azd |
SUIT-003 O2 ce-pce D020 | D020_ce1;ce1P;ce2Omy;ce3U;ce4Rot;ce5S;1Dig;1c;2Ama;3AsTm;4Azd |
SUIT-003 pH ce D067 | D067_ce1;ce1Glc;ce2Omy;ce3DG;ce4Ama |
SUIT-004 O2 pfi D010 | D010_1PM;2D;2c;3U;4S;5Rot;6Ama;7AsTm;8Azd |
SUIT-005 O2 pfi D011 | D011_1OctM;2D;2c;3P;4S;5U;6Rot;7Ama;8AsTm;9Azd |
SUIT-006 AmR mt D048 | D048_1PM;2D;3Omy;4U;5Ama |
SUIT-006 Fluo mt D034 | D034_1PM;2D;3Omy;4U;5Ama |
SUIT-006 MgG ce-pce D085 | D085_ce1;1Dig;1PM;2D;3Cat;4U;5Ama |
SUIT-006 MgG mt D055 | D055_1PM;2D;3Cat;4U;5Ama |
SUIT-006 O2 ce-pce D029 | D029_ce1;1Dig;1PM;2D;2c;3Omy;4U;5Ama |
SUIT-006 O2 mt D022 | D022_1SRot;2D;2c;(3Omy);4U;5Ama |
SUIT-006 O2 mt D047 | D047_1PM;2D;2c;3Omy;4U;5Ama |
SUIT-006 Q ce-pce D073 | D073_ce1;1Dig;1Q2;1Rot;1S;2D;(3Omy);4U;5Anox;6Ama.png |
SUIT-006 Q mt D071 | D071_mt;1Q2;1Rot;1S;2D;(3Omy);4U;5Anox;6Ama.png |
SUIT-007 O2 ce-pce D030 | D030_ce1;1Dig;1G;2D;2c;3M;4U;5Ama |
SUIT-008 O2 ce-pce D025 | D025_ce1;1Dig;1PM;2D;2c;3G;4S;5U;6Rot;7Ama;8AsTm;9Azd |
SUIT-008 O2 mt D026 | D026_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd |
SUIT-008 O2 pfi D014 | D014_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd |
SUIT-009 AmR ce-pce D019 | D019_ce1;1Dig;1S;2D;3P;4Rot;5Ama |
SUIT-009 AmR mt D021 | D021_1S;2D;3P;4Rot;5Ama |
SUIT-009 O2 ce-pce D016 | D016_ce1;1Dig;1S;2D;3P;4Rot;5Ama |
SUIT-009 O2 mt D015 | D015_1S;2D;3P;4Rot;5Ama |
SUIT-010 O2 ce-pce D008 | D008_ce1;ce2Rot;ce3S;ce4D;1Dig;1c |
SUIT-011 O2 pfi D024 | D024_1GM;2D;2c;3S;4U;5Rot;6Ama |
SUIT-012 O2 ce-pce D052 | D052_ce1;1Dig;1PM;2D;2c;3G;4U;5Ama |
SUIT-012 O2 mt D027 | D027_1PM;2D;2c;3G;4U;5Ama |
SUIT-013 AmR ce D023 | D023_ce1 |
SUIT-014 O2 pfi D042 | D042_1GM;2D;2c;3P;4S;5U;6Rot;7Ama |
SUIT-015 O2 pti D043 | D043_1OctM;2D;2c;3G;4P;5S;6U;7Rot;8Ama |
SUIT-016 O2 pfi D044 | D044_1OctM;2D;3G;4S;5Rot;6Omy;7U;8Ama |
SUIT-017 O2 mt D046 | D046_1OctM;2D;2c;3G;3c;4S;5U;6Rot;7Ama |
SUIT-017 O2 pfi D049 | D049_1OctM;2D;2c;3G;4S;5U;6Rot;7Ama |
SUIT-018 AmR ce-pce D068 | D068_ce1;1Dig;1GMS;2D;3Ama |
SUIT-018 AmR mt D031 | D031_mt;1GMS;2D;3Ama |
SUIT-018 AmR mt D040 | D040_mt;1GMS;2D;3Ama |
SUIT-018 AmR mt D041 | D041_mt;1GMS;2D;3Ama |
SUIT-018 O2 mt D054 | D054_mt;1GMS;2D;2c;3Ama |
SUIT-019 O2 pfi D045 | D045_1PalM;2D;2c;3Oct;4P;5G;6U;7S;8Rot;9Ama |
SUIT-020 Fluo mt D033 | D033_1PM;2D;3G;4S;5Rot;6Omy;7U;8Ama |
SUIT-020 O2 mt D032 | D032_1PM;2D;2c;3G;4S;5Rot;6Omy;7U;8Ama |
SUIT-021 Fluo mt D036 | D036_1GM;2D;3S;4Rot;5Omy;6U;7Ama |
SUIT-021 O2 mt D035 | D035_1GM;2D;2c;3S;4Rot;5Omy;6U;7Ama |
SUIT-022 O2 ce D051 | D051_ce1;ce2KCN;ce3SHAM |
SUIT-023 O2 ce D053 | D053_ce1;ce2SHAM;ce3KCN |
SUIT-024 O2 ce-pce D056 | D056_ce1;1Dig;1PM;2T;2D;3Omy;4Ama |
SUIT-025 O2 mt D057 | D057_1D;2M;3Oct;3c;4M;5P;6G;7S;8Rot;9Ama |
SUIT-026 AmR ce-pce D087 | D087_ce1;1Dig;1S;2Rot;3D;4Ama |
SUIT-026 AmR mt D064 | D064_1S;2Rot;3D;4Ama |
SUIT-026 AmR mt D077 | D077_1S;2Rot;3D;4Ama |
SUIT-026 O2 ce-pce D088 | D088_ce1;1Dig;1S;2Rot;3D;3c;4Ama |
SUIT-026 O2 mt D063 | D063_1S;2Rot;3D;3c;4Ama |
SUIT-027 O2 ce-pce D065 | D065_ce1;1Dig;1M;2D;3M;4P;5G |
SUIT-029 O2 mt D066 | D066_mt;1PM;2T;2D;2c;3Omy;4U;5G;6S;6U;7Rot;8Ama |
SUIT-031 O2 ce-pce D079 | D079_ce1;1Dig;1PM;2D;2c;3S;4Rot;5U;6Ama |
SUIT-031 O2 mt D075 | D075_1mt;1PM;2D;2c;3S;4Rot;5U;6Ama |
SUIT-031 Q ce-pce D074 | D074_ce1;1Dig;1Q2;1PM;2D;3S;4Rot;5U;6Anoxia;7Ama |
SUIT-031 Q mt D072 | D072_mt;1Q2;1PM;2D;3S;4Rot;5U;6Anox;7Ama |
Strategy
- Define substrates and inhibitors for each pathway state.
- Define coupling states.
Definition of states
Experimental conditions
- Temp
- O2
- Medium
- Oxygen level
- ADP kinetics
- Substrate kinetics
O2k configuration
- Amp: Amplex Red, TMRM, Ca Green, ...
- Pot
- Spectrophotometry
Define pathway states
- Pathway control states are defined for many substrate-inhibitor combinations in SUIT protocols, particularly: (N-pathway control state, S-pathway control state, NS-pathway control state). Titrations may performed in a single step, or in multiple steps for sequential analyis of different pathway control states.
- N: PM, GM, PGM, M, ..
- S: S(Rot), S, SM, ..
- NS: PS, GS, PMS, GMS, PGMS, ..
- CIV: AsTM
- F: Oct, Pal, ...
- Gp
Define coupling states
- The coupling control states require a complementary definition of an ET-pathway competent state.
- L, P, E (Uncouplers, U: FCCP, CCCP, DNP)
Define residual oxygen consumption
- ROX: Rot&Ama, Rot&Mna&Ama, Myx, Cn, Az, ..
Categories of SUIT protocols
SUIT protocol patterns
Examples of SUIT protocols
N; NS; S diametral
- Old nomenclature: CI + CI&II + CII diametral
- Respiratory states: NL -> NP -> NSP -> NSE -> SE -> ROX
- GM;D;S;U;Rot Innsbruck-Copenhagen protocol: GM;D;c;S;U;Rot ::Cyt c: added after ADP; cyt c added after S; ADP conc: _ _; ROX: Ama (Boushel 2007 Diabetologia)
- PMG;D;S;U;Rot Brain protocol: PGM;D;c;S;U;Rot::ADP conc: in mouse brain homogenate a final conc. of 2 mM ADP in the NS-OXPHOS state (NSP) is saturating (Sumbalova 2011 Abstract Bordeaux); ROX: Mna&Ama; Myx
N; N; NS; S diamteral
- Old nomenclature: CI + CI + CI&II + CII diamteral
- GM;D;P;S;U;Rot Innsbruck-Liege protocol 1: GM;D;c;P;S;U;Rot::Cytochrome c: early addition of c ensures comparability of all states in case of any c effect.
- Old nomenclature: CI + CI + CI&II + CII diamteral
- PM;D;G;S;U;Rot Innsbruck-Liege protocol 2: PM;D;c;G;S;U;Rot
F; FN; FNS; S diametral
- Old nomenclature: FAO + CI + CI&II + CII diametral
- Respiratory states: FL -> FP -> FNP -> FNSP -> FNSE -> SE -> ROX
- OctM;D;G;S;U;Rot Innsbruck training protocol: OctM;D;G;c;S;U;Rot::Pesta 2012 Methods Mol Biol, Pesta 2011 Am J Physiol Regul Integr Comp Physiol
- Respiratory states: FL -> FP -> FNP -> FNSP -> SP -> SL -> SE -> ROX
- OctM;D;G;S;Rot;Omy;U Greenland-Monte Rosa protocol: OctM;D;G;S;Rot;Omy;U;c::Cyt c: added after U. The absence of a significant c-effect shows the integrity of the outer mitochondrial membrane after fibre preparation and also shows the preservation of integrity over a 90-100 min incubation in the O2k (MiR05); Oxygen: oxygen limitation of flux was preventd by maintaining oxygen levels in the respirometer above air saturation (Pesta 2012 Methods Mol Biol); ROX: Mna&Ama
References and notes
MitoPedia concepts:
MiP concept,
SUIT concept