SUIT-006 O2 mt D022: Difference between revisions
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|description=[[File:1SRot;2D;2c;3U;4Ama.png|400px]][[File:SUIT-006 O2 mt D022 traces.png| | |description=[[File:1SRot;2D;2c;3U;4Ama.png|400px]][[File:SUIT-006 O2 mt D022 traces.png|600px]] | ||
|info='''A''' | |info='''A''' | ||
|application=O2 | |application=O2 |
Revision as of 18:29, 21 February 2019
Description
Abbreviation: S
Reference: A
SUIT number: D022_1SRot;2D;2c;3U;4Ama
O2k-Application: O2
- SUIT-category: S
- SUIT protocol pattern: linear coupling control protocol 1SRot;2D;2c;3U;4Ama
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Krumschnabel 2014 Methods Enzymol | 2014 | Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. https://doi.org/10.1016/B978-0-12-416618-9.00009-1 | Mouse | Nervous system |
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1SRot | SL | S | CII | 1SRot
|
2D | SP | S | CII | 1SRot;2D
|
2c | ScP | S | CII | 1SRot;2D;2c
|
(3Omy) | ScL(Omy) | 1SRot;2D;2c;(3Omy)
| ||
4U | SE | S | CII | 1SRot;2D;2c;4U
|
5Ama | ROX | 1SRot;2D;2c;4U;5Ama
|
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- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- This protocol is indicated when the aim is to analyse coupling control in mtprep (see MitoPedia: Sample preparations) at Succinate pathway control state.
- + The combination of succinate with rotenone, that inhibits complex I, provides the best coupling control protocol specific for S-pathway, avoiding reverse electron transfer (RET).
- + Reasonable duration of the experiment.
- + This protocol can be extended with the Complex IV module.
- - This protocol does not include internal ET-pathway control steps.
- - Careful washing is required after the experiment to avoid carry-over of uncoupler and inhibitors.
Compare SUIT protocols
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry