Dechandt 2016 Anal Biochem: Difference between revisions
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|title=Dechandt CRP, Couto-Lima CA, Alberici LC (2016) Triglyceride depletion of brown adipose tissue enables analysis of mitochondrial respiratory function in permeabilized biopsies. Anal Biochem 515:55-60. | |title=Dechandt CRP, Couto-Lima CA, Alberici LC (2016) Triglyceride depletion of brown adipose tissue enables analysis of mitochondrial respiratory function in permeabilized biopsies. Anal Biochem 515:55-60. | ||
|info=[https://www.ncbi.nlm.nih.gov/pubmed/27717856 PMID: 27717856] | |info=[https://www.ncbi.nlm.nih.gov/pubmed/27717856 PMID: 27717856] | ||
|authors=Dechandt CRP, Couto-Lima CA, Alberici | |authors=Dechandt CRP, Couto-Lima CA, Alberici Luciane Carla | ||
|year=2016 | |year=2016 | ||
|journal=Anal Biochem | |journal=Anal Biochem | ||
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|keywords=Biopsy, Brown adipose tissue, Mitochondria, Oroboros, Respiration | |keywords=Biopsy, Brown adipose tissue, Mitochondria, Oroboros, Respiration | ||
|editor=[[Plangger M]], | |editor=[[Plangger M]], | ||
|mipnetlab=BR Ribeirao Preto Alberici LC | |||
}} | }} | ||
{{Labeling | {{Labeling |
Latest revision as of 11:16, 7 March 2020
Dechandt CRP, Couto-Lima CA, Alberici LC (2016) Triglyceride depletion of brown adipose tissue enables analysis of mitochondrial respiratory function in permeabilized biopsies. Anal Biochem 515:55-60. |
Dechandt CRP, Couto-Lima CA, Alberici Luciane Carla (2016) Anal Biochem
Abstract: The research on mitochondrial functions in adipocytes has increasingly evidenced that mitochondria plays an important role in the onset and/or progression of obesity and related pathologies. Mitochondrial function in brown adipose tissue (BAT) has been classically assessed by measuring either the levels/activity of mitochondrial enzymes, or the respiration in isolated mitochondria. Isolation of mitochondria is not advantageous because it demands significant time and amount of tissue and, as tissue homogenates, disrupts biochemical and physical connections of mitochondria within the cell. Here, we described a new and efficient protocol to analyze the mitochondrial respiratory states in BAT biopsies that relies on intracellular triglyceride depletion followed by tissue permeabilization. In addition to minimizing tissue requirements to โผ17 mg wet weight, the proposed protocol enabled analysis of all mitochondrial respiratory states, including phosphorylation (OXPHOS), no-phosphorylation (LEAK), and uncoupled (ETS) states, as well as the use of substrates for complex I, complex II, and cytochrome c; together, these features demonstrated mitochondrial integrity and validated the preparation efficacy. Therefore, the protocol described here increases the possibilities of answering physiological questions related to small BAT regions of human and animal models, which shall help to unravel the mechanisms that regulate mitochondrial function in health and disease. โข Keywords: Biopsy, Brown adipose tissue, Mitochondria, Oroboros, Respiration โข Bioblast editor: Plangger M โข O2k-Network Lab: BR Ribeirao Preto Alberici LC
Labels: MiParea: Respiration, Instruments;methods
Organism: Rat
Tissue;cell: Fat
Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, ROX
HRR: Oxygraph-2k
Labels, 2018-09