Template talk:Next oroboros ecosystem agenda: Difference between revisions
(→Next) |
|||
Line 32: | Line 32: | ||
|''' Is there a difference between [[State 3]] and [[OXPHOS capacity]]? '''<br> | |''' Is there a difference between [[State 3]] and [[OXPHOS capacity]]? '''<br> | ||
Yes, OXPHOS capacity is measured at kinetically-saturating concentrations of ADP, while in state 3 usually "high" concentrations of ADP are used, which can be 10-fold lower than kinetically-saturating ADP concentrations.<br> | Yes, OXPHOS capacity is measured at kinetically-saturating concentrations of ADP, while in state 3 usually "high" concentrations of ADP are used, which can be 10-fold lower than kinetically-saturating ADP concentrations.<br> | ||
- »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC: Mitochondrial physiology]''' - 2.5 Coupling states and respiratory states, OXPHOS and 2.6.3 Classical terminology for isolated mitochondria, State 3 | |||
|} | |||
Suggestion 2: | |||
:{| | |||
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]] | |||
|''' Is there a difference between [[State 3]] and [[OXPHOS capacity]]? '''<br> | |||
Yes, state 3 is measured with "high" concentrations of ADP, which can be 10-fold lower than kinetically-saturating ADP concentrations that is measured in OXPHOS capacity.<br> | |||
- »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC: Mitochondrial physiology]''' - 2.5 Coupling states and respiratory states, OXPHOS and 2.6.3 Classical terminology for isolated mitochondria, State 3 | - »'''[https://www.bioenergetics-communications.org/index.php/BEC_2020.1_doi10.26124bec2020-0001.v1 BEC: Mitochondrial physiology]''' - 2.5 Coupling states and respiratory states, OXPHOS and 2.6.3 Classical terminology for isolated mitochondria, State 3 | ||
|} | |} |
Revision as of 14:40, 31 August 2020
- Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
{| | logos | title in bold <br> - link |}
e.g.,
Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?
- »Instrumental background« and »TIP2k Filter Papers«
- See the scheme:
Bioblast alert 2020
Prep for circular done here: Talk:Bioblast alert 2020
MitoPedia alert
Next
- Sep-10 Thr
Is there a difference between State 3 and OXPHOS capacity?
Yes, OXPHOS capacity is measured at kinetically-saturating concentrations of ADP, while in state 3 usually "high" concentrations of ADP are used, which can be 10-fold lower than kinetically-saturating ADP concentrations.
- »BEC: Mitochondrial physiology - 2.5 Coupling states and respiratory states, OXPHOS and 2.6.3 Classical terminology for isolated mitochondria, State 3
Suggestion 2:
Is there a difference between State 3 and OXPHOS capacity?
Yes, state 3 is measured with "high" concentrations of ADP, which can be 10-fold lower than kinetically-saturating ADP concentrations that is measured in OXPHOS capacity.
- »BEC: Mitochondrial physiology - 2.5 Coupling states and respiratory states, OXPHOS and 2.6.3 Classical terminology for isolated mitochondria, State 3
- Sep-17 Thr
Did you know that the free ROUTINE activity allows you to indirectly measure ATP production in living cells?
The free ROUTINE activity is ROUTINE respiration corrected for LEAK respiration which is available for phosphorylation of ADP to ATP in living cells.
- »SUIT-003 O2 ce D009
Suggestion 2 (Luiza):
Did you know that the free ROUTINE activity allows you to assess the respiratory activity available for phosphorylation of ADP to ATP in living cells?
SUIT protocols from the SUIT-003 family will guide you through the titrations needed to analyse free ROUTINE activity.
- »SUIT-003 O2 ce D009« and »SUIT-003 O2 ce-pce D020«
O2k-Brief alert
Next
- Sep-04 Fr (pending publication of Luiza's video communication)
Measuring ATP-ADP exchange via adenine nucleotide translocase (ANT) using the fluorophore Magnesium Green (MgG)
- from the O2k-Network »HU Budapest Chinopoulos C«
- Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. »Bioblast link«
- from the O2k-Network »HU Budapest Chinopoulos C«
O2k-Publications alert
Author names of O2k-Publications/ Brief for the Agenda vs Bioblast
- 1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: Template alerts.
- Example: Oroboros Ecosystem Agenda example
- This means that we have to edit manually the author names within the O2k-Publications/ Brief alerts (i.e., Antunes Diana (from Bioblast) to Antunes D (to Agenda entries)) if they are long or copy from the gray box.
- 2.Bioblast: SOP: [1] – examples are available using both Surname and First name with long version (e.g., Antunes Diana). If in Bioblast the authors are with short surname in the links please update it to complete surname, guidelines are below.
- 1.Oroboros Ecosystem Agenda: SOP: Surname with long version and First name with short version. Please, see: Template alerts.
Guidelines to update a reference
- When creating an O2k-Publication/Brief alert, it is the responsibility of the author to check if the reference is accordingly to the correct guidelines:
- Correct format for a person called “First Name Surname”:
- In the gray box should be “Surname FN” - From where references to be used as O2k-Publication/Brief alerts are copied from.
- In the links should be “ Surname First N”
- How to edit:
- A) Check if all authors with profiles in Bioblast (blue links) have their profiles with full name. If they have, proceed to next step (B). If not, you need to move it following the steps “How to move a profile” below.
- B) Click in “Edit with form”.
- C) The gray box is edited in “Reference” and the links are changed in “Author(s)”. Change it accordingly and click in “save page” at the end of the page.
- How to edit:
How to move a profile (instructions sent by Marija):
So let’s take for example my page: Beno M [2]
- As you can see there is a redirect from Beno Marija to Beno M. What has to be done is to switch the content of those pages. Here are the steps:
- 1. Go to e.g. “Beno M” on edit
- 2. Click on “Move”
- 3. Change the new title to the full name of the person. In my case it will be Beno M to Beno Marija
- As you can see there is a redirect from Beno Marija to Beno M. What has to be done is to switch the content of those pages. Here are the steps:
- 4. Now it should look like that:
- 5. Click on “Move” and leave all the ticked boxes
- 6.Done
- After that you can continue in step B.
- If you have any doubt you can contact Cristiane, Marija or Mario.
Next
- Sep-21 Mo - World Alzheimer's Day
Sentence
- from the O2k-Network »link«
- Reference »Bioblast link«
- Sep-29 Tu - World Heart Day
Sentence
- from the O2k-Network »link«
- Reference »Bioblast link«
O2k-Open Support alert
Next
- While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
Removed for correction
Do you perform the leaky chamber test after chamber assembly?
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- »Leaky chamber test« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«
- Aug-17 Mo
Have you observed an 'O2 slope neg.' > 4 pmol∙s−1∙mL−1 in the closed 2 mL chamber at air saturation without a biological sample?
This may be due to biological contamination of the medium or the O2k-Chamber.
For further details on how to clean the O2k-Chamber properly, see:
- » Cleaning of the O2k-Chamber«
O2k-Open Support alert circulars
In preparation
For discussion
Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions: - Proven -80 °C solid stability for more than 3 years (3 years 7 months)
- Proven -20 °C solid stability for 1 year
- Proven DMSO stability at room temperature for 1 week
- Proven DMSO stability at -20 °C for 6 months (ongoing)
2021
- 2021-01-06 https://www.jstor.org/stable/1706346?seq=1
Postponed events
- Abstracts deadline 10th Int. CeBiTec Research Conference Advances in Industrial Biotechnology: Prospects and challenges for the development of algal biotechnology
- Notification to oral and poster presenters - »MitoEAGLE Summit Obergurgl 2020« (2020 Nov 04-07) and »MiPschool Obergurgl 2020« (2020 Nov 09-11)
Bioenergetics Vienna 2020
IOC Schroecken AT
- Registration and late Abstract Deadline - »MitoEAGLE Summit Obergurgl 2020« and »MiPschool Obergurgl 2020«