Ocloo 2017 J Appl Pharmac Sc

Ocloo A, Kingsford-Adaboh R, Murray AJ (2017) Inhibition of mitochondrial respiratory chain activity by O, O-Dimethyl- and 4-O- Methyl- Alpinumisoflavones. J Appl Pharmac Sc 7:95-100.

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Ocloo A, Kingsford-Adaboh R, Murray AJ (2017) J Appl Pharmac Sc

Abstract: Although the molluscicidal activity of crude dichloromethane extract of Millettia thonningii, known to be rich in alpinumisoflavones was attributed to its inhibition of isolated rat liver mitochondrial complex I activity, the effects of the constituent alpinuisoflavones on mitochondrial function have not been reported. The present study, therefore investigated the effect of O, O-dimethyl-alpinumisoflavone, a constituent of the crude dichloromethane extract of M. thonningii seeds and 4'-O-methyl-alpinumsioflavone, a naturally occurring alpinumisoflavone in Lonchocarpus glabrescens in isolated rat liver mitochondria and permeabilised mouse heart fibers using substrate-inhibitor titrations. The O, O-dimethyl-alpinumisoflavone inhibited State 3 respirations supported by glutamate/malate (complex I), succinate (complex II) and Ascorbate/TMPD (complex IV) in the permeabilised cardiac tissues but inhibited only the State 3 respiration supported by glutamate (complex I) in the isolated rat liver mitochondria. The 4'-O-methyl-alpinumsioflavone on the other hand inhibited State 3 respirations supported by all the substrates in both the isolated rat liver mitochondria and the permeabilised cardiac tissues. Thus, whilst O, O-dimethyl-alpinumisoflavone, a naturally occurring phyto-compound in the extract of M. thonningii seeds inhibits mitochondrial respiratory chain activity essentially at complex I, its derivative 4'-O-methyl-alpinumisoflavone, a naturally occurring phytocompound in Lonchocarpus glabrescens inhibits mitochondrial respiratory complexes I, II and IV. • Keywords: Mitochondrial respiratory complexes, Inhibition, Alpinumisoflavones • Bioblast editor: Kandolf G • O2k-Network Lab: UK Cambridge Murray AJ

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Organism: Rat  Tissue;cell: Liver  Preparation: Isolated mitochondria