MiPNet19.15 IOC97: Difference between revisions
No edit summary |
Beno Marija (talk | contribs) No edit summary |
||
(46 intermediate revisions by 7 users not shown) | |||
Line 1: | Line 1: | ||
{{ | {{OROBOROS header page name}} | ||
{{Publication | {{Publication | ||
|title=[[Image:O2k- | |title=[[Image:O2k-Network.png|right|60px|link=O2k-Network|O2k-Network]] | ||
'''Lausanne CH''', 2014 Sep. 25-26 | '''Lausanne CH''', 2014 Sep. 25-26 Oroboros O2k-Workshop on HRR and O2k-Fluorometry, '''IOC97.''' | ||
|info=[[ | |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/1/1d/MiPNet19.15_IOC97_2014-09_Lausanne.pdf |Bioblast pdf]] | ||
|authors= | |authors=Oroboros | ||
|year=2014-09-25 | |year=2014-09-25 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=[[Image:O2k-Workshops.png|left|130px|link= | |abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops|O2k-Workshops]] | ||
'''97th | '''97th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry''' | ||
|mipnetlab=CH Lausanne Sandi C, AT Innsbruck | |mipnetlab=CH Lausanne Sandi C, AT Innsbruck Oroboros | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|instruments=Oxygraph-2k | |||
|additional=ORO, IOC, 2014, O2k-Network | |||
|instruments=Oxygraph-2k | |||
|additional=ORO, IOC, 2014, | |||
}} | }} | ||
'''»''' [[ | '''»''' [[MitoPedia: Respiratory states]] | ||
[[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image: | [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET capacity|ETS]] [[Image:L.jpg|link=LEAK respiration|LEAK]] [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]] | ||
__TOC__ | __TOC__ | ||
== Lecturer == | == Lecturer == | ||
[[File:Gnaiger Erich.jpg | [[File:Gnaiger Erich.jpg|80px|Erich Gnaiger]] | ||
[[Gnaiger Erich]], CEO, | [[Gnaiger Erich]], CEO, Oroboros Instruments | ||
== Programme == | |||
[[Media:MiPNet19.15_IOC97_2014-09_Lausanne.pdf |'''Programme MiPNet19.15.pdf''']] | |||
=== Thursday, September 25 === | |||
09:00 – 10:30 A new Power-O2k Lab of Prof. Carmen Sandi: 4 O2k. | |||
::::* Instrumental setup, chamber assembly, OroboPOS – sensor service, quality control (group Carmen Sandi). | |||
10:30 ''Break – Registration of external participants'' | |||
11:00 – 11:15 Erich Gnaiger: | |||
:::: Welcome to external participants. Oroboros Instruments and perspectives of mitochondrial physiology. | |||
11:15 – 11:30 Introduction of participants: who is who? | |||
11:30 – 12:30 Basic and new features of DatLab 6. | |||
::::* Oxygen calibration. | |||
::::* Oxygen flux and instrumental tests. | |||
12:30 Lunch | |||
13:30 – 15:30 Experimental design. | |||
::::* Cell ergometry: from intact cells to mt-preparations. | |||
::::* A challenge for simultaneous measurements of respiration and mt-membrane potential: solving a puzzle. | |||
15:30 Break | |||
16:00 – 17:30 Titration-Injection microPump TIP2k: | |||
:::: From automatic instrumental background tests to steady-state control in respiratory experiments. | |||
17:30 – 18:00 Q&A session on HRR and OXPHOS analysis | |||
:::: Design of experimental protocol - day 2. | |||
=== Friday, September 26 === | |||
09:00 – 10:30 Experiment | |||
:::: HRR and O2k-Fluorometry – respiration and H2O2 production. | |||
10:30 Break | |||
11:00 – 12:30 Experiment continued. | |||
12:30 Lunch | |||
13:15 – 15:30 DatLab analysis. | |||
15:30 Break | |||
16:00 – 16:30 Trouble shooting. | |||
16:30 – 17:30 Normalization of mt-respiration: flux control states and flux control steps. | |||
17:30 - 18:00 Feedback – conclusions – stay connected as a MiPNet Lab. | |||
=== Venue === | === Venue === | ||
Laboratory of Behavioral Genetics | |||
Brain Mind Institute | |||
Ecole Polytechnique Federale de Lausanne | |||
Auditorium/Meeting room | |||
CH-1015 Lausanne | |||
Contact: Prof. Carmen Sandi, Director. Email: [email protected] | |||
Line 47: | Line 98: | ||
Oroboros Instruments | |||
high-resolution respirometry | high-resolution respirometry | ||
Line 58: | Line 109: | ||
Fax: +43 512 566796 20 | Fax: +43 512 566796 20 | ||
== Participants == | |||
{| class="wikitable" | |||
|- | |||
! Name | |||
! Institution | |||
|- | |||
|[[Canto_AC]] (Thursday, Friday) | |||
|'''[[CH Lausanne Canto C]]:''' Nestlé Institute of Health Sciences SA, EPFL | |||
|- | |||
|[[Eggimann Sandra]] | |||
|'''[[CH Bern Nuoffer JM]]:''' Universitätsinstitut für klinische Chemie, Inselspital Bern | |||
|- | |||
|'''Grosse Jocelyn''' (Thursday) | |||
|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL | |||
|- | |||
|[[Hahn Dagmar]] | |||
|'''[[CH Bern Nuoffer JM]]:''' Universitätsinstitut für klinische Chemie, Inselspital Bern | |||
|- | |||
|'''[[Hollis Fiona]]''' (Thursday afternoon and Friday) | |||
|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL | |||
|- | |||
|'''Huzard Damien''' (Thursday morning and Friday morning) | |||
|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL | |||
|- | |||
|'''Meng Kévin''' (Thursday afternoon and Friday morning) | |||
|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL | |||
|- | |||
|'''Kulkarni Sameer''' | |||
|'''[[CH Lausanne Canto C]]:''' Nestlé Institute of Health Sciences SA, EPFL | |||
|- | |||
|'''Papilloud Aurélie''' (Thursday and Friday) | |||
|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL | |||
|- | |||
|'''Ratajczak Joanna''' (Thursday afternoon and Friday) | |||
|'''[[CH Lausanne Canto C]]:''' Nestlé Institute of Health Sciences SA, EPFL | |||
|- | |||
|'''Riccio Orbicia''' (Thursday afternoon and Friday) | |||
|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL | |||
|- | |||
|'''[[Sandi Carmen]]''' | |||
|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL | |||
|- | |||
|'''Walker Sophie''' (Thursday) | |||
|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL | |||
|- | |||
|'''Zanoletti Olivia''' (Thursday and Friday) | |||
|'''[[CH Lausanne Sandi C]]:''' Brain Mind Institute, EPFL | |||
|} | |||
Line 63: | Line 165: | ||
<big>'''Key references'''</big> | <big>'''Key references'''</big> | ||
[[Image:O2k-Publications.jpg|left|50px|link= | [[Image:O2k-Publications.jpg|left|50px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]] | ||
: | : Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. [[Gnaiger_2008_POS |»Bioblast Access]] | ||
:* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. [[Pesta 2012 Methods Mol Biol |»Bioblast Access]] | |||
:* Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small | |||
<big>'''Introduction and O2k-Core Manual'''</big> | <big>'''Introduction and O2k-Core Manual'''</big> | ||
[[Image:O2k-Manual.jpg|left|30px|link= | [[Image:O2k-Manual.jpg|left|30px|link=O2k-Manual|O2k-Manual]] | ||
: [[Media:MiPNet19.01 O2k-Core Manual.pdf|'''»O2k-Core Manual.pdf''']] | |||
<big>'''HRR and O2k-Fluorometry'''</big> | <big>'''HRR and O2k-Fluorometry'''</big> | ||
[[Image:O2k-Fluorometer.JPG|60px|left|link=O2k-Fluorometer|O2k-Fluorometer]] | |||
: Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. [[Krumschnabel 2014 Methods Enzymol |»Pubmed 24862266]] | |||
* »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]''' | :* »[[MiPNet17.05 O2k-Fluo LED2-Module |Manual: O2k-Fluo LED2-Module]] | ||
:* »[[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]''' | |||
<big>'''Mitochondrial pathways'''</big> | <big>'''Mitochondrial pathways'''</big> | ||
[[Image:O2k-Protocols.jpg|left|40px|link=http://oroboros.at/?O2k-Protocols|O2k-Protocols]] | [[Image:O2k-Protocols.jpg|left|40px|link=http://oroboros.at/?O2k-Protocols|O2k-Protocols]] | ||
Gnaiger E ( | : Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck: 80 pp. [[Gnaiger 2014 MitoPathways |»Open Access]] | ||
[[Image:MitoGlobal.jpg|right|80px|link= | [[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] | ||
O2k-Workshops are listed as [[MitoGlobal Events]]. | O2k-Workshops are listed as [[MitoGlobal Events]]. | ||
[[Category:O2k-Workshops]] | [[Category:O2k-Workshops]] |
Latest revision as of 15:13, 8 June 2020
MiPNet19.15 IOC97
Lausanne CH, 2014 Sep. 25-26 Oroboros O2k-Workshop on HRR and O2k-Fluorometry, IOC97. |
Oroboros (2014-09-25) Mitochondr Physiol Network
Abstract:
97th Oroboros O2k-Workshop on high-resolution respirometry and O2k-Fluorometry
• O2k-Network Lab: CH Lausanne Sandi C, AT Innsbruck Oroboros
Labels:
HRR: Oxygraph-2k
ORO, IOC, 2014, O2k-Network
» MitoPedia: Respiratory states
Lecturer
Gnaiger Erich, CEO, Oroboros Instruments
Programme
Programme MiPNet19.15.pdf
Thursday, September 25
09:00 – 10:30 A new Power-O2k Lab of Prof. Carmen Sandi: 4 O2k.
- Instrumental setup, chamber assembly, OroboPOS – sensor service, quality control (group Carmen Sandi).
10:30 Break – Registration of external participants
11:00 – 11:15 Erich Gnaiger:
- Welcome to external participants. Oroboros Instruments and perspectives of mitochondrial physiology.
11:15 – 11:30 Introduction of participants: who is who?
11:30 – 12:30 Basic and new features of DatLab 6.
- Oxygen calibration.
- Oxygen flux and instrumental tests.
12:30 Lunch
13:30 – 15:30 Experimental design.
- Cell ergometry: from intact cells to mt-preparations.
- A challenge for simultaneous measurements of respiration and mt-membrane potential: solving a puzzle.
15:30 Break
16:00 – 17:30 Titration-Injection microPump TIP2k:
- From automatic instrumental background tests to steady-state control in respiratory experiments.
17:30 – 18:00 Q&A session on HRR and OXPHOS analysis
- Design of experimental protocol - day 2.
Friday, September 26
09:00 – 10:30 Experiment
- HRR and O2k-Fluorometry – respiration and H2O2 production.
10:30 Break
11:00 – 12:30 Experiment continued.
12:30 Lunch
13:15 – 15:30 DatLab analysis.
15:30 Break
16:00 – 16:30 Trouble shooting.
16:30 – 17:30 Normalization of mt-respiration: flux control states and flux control steps.
17:30 - 18:00 Feedback – conclusions – stay connected as a MiPNet Lab.
Venue
Laboratory of Behavioral Genetics
Brain Mind Institute
Ecole Polytechnique Federale de Lausanne
Auditorium/Meeting room
CH-1015 Lausanne
Contact: Prof. Carmen Sandi, Director. Email: [email protected]
Registration and general information
Oroboros Instruments
high-resolution respirometry
Schöpfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Participants
Name | Institution |
---|---|
Canto_AC (Thursday, Friday) | CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL |
Eggimann Sandra | CH Bern Nuoffer JM: Universitätsinstitut für klinische Chemie, Inselspital Bern |
Grosse Jocelyn (Thursday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Hahn Dagmar | CH Bern Nuoffer JM: Universitätsinstitut für klinische Chemie, Inselspital Bern |
Hollis Fiona (Thursday afternoon and Friday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Huzard Damien (Thursday morning and Friday morning) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Meng Kévin (Thursday afternoon and Friday morning) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Kulkarni Sameer | CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL |
Papilloud Aurélie (Thursday and Friday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Ratajczak Joanna (Thursday afternoon and Friday) | CH Lausanne Canto C: Nestlé Institute of Health Sciences SA, EPFL |
Riccio Orbicia (Thursday afternoon and Friday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Sandi Carmen | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Walker Sophie (Thursday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Zanoletti Olivia (Thursday and Friday) | CH Lausanne Sandi C: Brain Mind Institute, EPFL |
Recommended reading
Key references
- Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access
- Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopsies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access
Introduction and O2k-Core Manual
HRR and O2k-Fluorometry
- Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. »Pubmed 24862266
Mitochondrial pathways
- Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck: 80 pp. »Open Access
O2k-Workshops are listed as MitoGlobal Events.