Carbonyl cyanide m-chlorophenyl hydrazone: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''C: CCCP''' (Carbonyl cyanide 3-chlorophenylhydrazone, C<sub>9</sub>H<sub>5</sub>ClN<sub>4</sub>O); Sigma C 2759, 100 mg, store at -20 °C: FW = 204.62 | '''C: CCCP''' (Carbonyl cyanide 3-chlorophenylhydrazone, C<sub>9</sub>H<sub>5</sub>ClN<sub>4</sub>O); Sigma C 2759, 100 mg, store at -20 °C: FW = 204.62 | ||
Revision as of 11:29, 5 June 2013
- high-resolution terminology - matching measurements at high-resolution
Carbonyl cyanide m-chlorophenyl hydrazone
Description
[[Description::Carbonyl cyanide m-chloro phenyl hydrazone (C9H5ClN4; FW = 204.62) is a chemical inhibitor of oxidative phosphorylation and causes an uncoupling (uncoupler) of the proton gradient that is established during the normal activity of electron carriers in the electron transport system.]]
Abbreviation: CCCP
MitoPedia topics:
Uncoupler
Labels:
Regulation: Respiration; OXPHOS; ETS Capacity
HRR: Chemicals; Media
Application in HRR
C: CCCP (Carbonyl cyanide 3-chlorophenylhydrazone, C9H5ClN4O); Sigma C 2759, 100 mg, store at -20 °C: FW = 204.62
Caution: Toxic!
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 1 mM stock solution (dissolved in DMSO):
- 1) weigh 1.02 mg of CCCP into a glass vial
- 2) dissolve in 5 ml DMSO
- 3) divide into 0.2 ml portions OR store in 5 ml glass vial and use subsamples during experiments to avoid contamination of the stock solution
- 3) store in glass vials at -20 °C
- Titration volume: 1 µl steps using a 10 µl syringe
- Final conc. in 2 ml O2k-chamber: 0.5 µM steps
