Rasmussen 1997 Mol Cell Biochem: Difference between revisions
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{{Publication | {{Publication | ||
|title=Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria of integrity, and assays with reference to tissue function. Mol Cell Biochem 174: 55-60. | |title=Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria of integrity, and assays with reference to tissue function. Mol Cell Biochem 174:55-60. | ||
|authors=Rasmussen HN, Rasmussen UF | |authors=Rasmussen HN, Rasmussen UF | ||
|year=1997 | |year=1997 | ||
Line 9: | Line 9: | ||
|taxonomic group=Birds | |taxonomic group=Birds | ||
|tissues=Skeletal muscle | |tissues=Skeletal muscle | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|enzymes=Complex I, Complex II; | |enzymes=Complex I, Complex II;succinate dehydrogenase, Complex V;ATP synthase | ||
|topics=Substrate; Glucose; TCA Cycle | |topics=Substrate; Glucose; TCA Cycle | ||
}} | }} |
Revision as of 16:56, 11 February 2015
Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria of integrity, and assays with reference to tissue function. Mol Cell Biochem 174:55-60. |
Rasmussen HN, Rasmussen UF (1997) Mol Cell Biochem
Abstract: Mitochondria prepared in small scale from skeletal muscle were studied with respiration measurements and low temperature spectroscopy. The method of preparation was developed for 25-100 mg tissue with pigeon breast muscle as model organ. The yield was 40%. Data collected during the developmental work were used to evaluate criteria of mitochondrial quality. The cytochrome c conservation, i.e. cytochrome c per mitochondrial quantity in the preparation relative to that in the tissue, is a most useful test parameter. It is bounded between 0-100%. Proportionality between the state 3 rate and the cytochrome c conservation was not rejected by statistical tests. The respiratory control ratio (RCR) was also highly correlated to the cytochrome c conservation. These correlations might be extrapolated to 100% conservation to give hypothetical tissue values. The cause for the correlations is discussed. The P/O ratio showed only weak dependence on the cytochrome c conservation and the state 4 rate showed no dependence. Other, rather insensitive test parameters are also discussed. The pigeon breast muscle mitochondria isolated by the final method showed cytochrome c conservation of 73 +/- 9% (n = 16). They are compared with pig biceps femoris mitochondria prepared by the same method. The two types of mitochondria show many similarities. Some differences may be explained by a different amount of inner mitochondrial membrane relative to mitochondrial protein. The pig tissue contains ten times less mitochondrial protein than the pigeon tissue does.
Labels:
Tissue;cell: Skeletal muscle Preparation: Isolated mitochondria Enzyme: Complex I, Complex II;succinate dehydrogenase, Complex V;ATP synthase Regulation: Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.