Succinate: Difference between revisions
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|info=[[MiPNet11.09]], [[MiPNet12.11]] | |info=[[MiPNet11.09]], [[MiPNet12.11]] | ||
|type=Respiration | |type=Respiration | ||
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|mitopedia topic=Substrate and metabolite | |||
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|discipline=Mitochondrial Physiology | |discipline=Mitochondrial Physiology | ||
|enzymes=Complex II; Succinate Dehydrogenase | |enzymes=Complex II; Succinate Dehydrogenase | ||
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|instruments=Chemicals; Media | |instruments=Chemicals; Media | ||
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
Revision as of 14:39, 9 March 2011
- high-resolution terminology - matching measurements at high-resolution
Succinate
Description
[[Description::Succinate2- is formed in the TCA cycle, and is a substrate of CII, reacting to fumarate and feeding electrons into the Q-junction through flavin adenine dinucleotide (FADH2). Succinate supports electron flux exclusively through Complex II via FADH2.]]
Abbreviation: S
Reference: [[Info::MiPNet11.09, MiPNet12.11]]
MitoPedia topics:
Substrate and metabolite
Labels:
Enzyme: Complex II; Succinate Dehydrogenase Regulation: Substrate; Glucose; TCA Cycle
HRR: Chemicals; Media
Application in HRR
S: Succinate (Succinate disodium salt, hexahydrate, C4H404Na2); Sigma S 2378, 100 g, store at R.T.): FW = 270.1
Preparation of 1 M stock solution (dissolved in H2O):
- 1) weigh 1.3505 g of Succinate and dissolve in 3 ml H2O
- 2) check pH and adjust to 7.0 if necessary with 1 N HCl (usually the pH is 7 without any adjustment)
- 3) transfer to 5 mL volumetric glass flask and adjust the final volume to 5 ml
- 4) divide into 0.2 ml portions
- 5) store frozen at -20 °C
Oxygraph-2k Manual Titrations: MiPNet09.12
- Titration volume: 20 µl using a 50 µl syringe
- Final conc. in 2 ml O2k-chamber: 10 mM
