ATP: Difference between revisions
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
'''T: ATP''' (Adenosine 5'-triphosphate disodium salt hydrate, C<sub>10</sub>H<sub>14</sub>N<sub>5</sub>O<sub>13</sub>P<sub>3</sub>Na<sub>2</sub>* (H<sub>2</sub>O)<sub>x</sub>); Sigma A 2383, 5 g, store at R.T.): FW = 551.14.Β Alternative source:Β MerckΒ 1191-1GM (Calbiochem; FW = 551.2; store at -20 Β°C). | '''T: ATP''' (Adenosine 5'-triphosphate disodium salt hydrate, C<sub>10</sub>H<sub>14</sub>N<sub>5</sub>O<sub>13</sub>P<sub>3</sub>Na<sub>2</sub>* (H<sub>2</sub>O)<sub>x</sub>); Sigma A 2383, 5 g, store at R.T.): FW = 551.14 (anhydrous basis).Β Alternative source:Β MerckΒ 1191-1GM (Calbiochem, Adenosine 5β²-Triphosphate, Disodium Salt; FW = 551.2; store at -20 Β°C). | ||
Revision as of 11:03, 16 May 2012
Description
Adenosine 5β-triphosphate hydrate(T), C10H14N5O13P3Na2.
Abbreviation: T
Reference: MiPNet03.02; MiPNet09.12
MitoPedia topics:
Substrate and metabolite
Application in HRR
T: ATP (Adenosine 5'-triphosphate disodium salt hydrate, C10H14N5O13P3Na2* (H2O)x); Sigma A 2383, 5 g, store at R.T.): FW = 551.14 (anhydrous basis). Alternative source: Merck 1191-1GM (Calbiochem, Adenosine 5β²-Triphosphate, Disodium Salt; FW = 551.2; store at -20 Β°C).
Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
Preparation of 500 mM ATP stock solution (dissolved in H2O):
- 1) weigh 501.3 mg of ADP (FW = 501.3) = (1 mmol ADP)
- 2) add 1.2 ml H2O (ADP is not dissolved at this stage.
- 3) neutralize with 5 N KOH (approx. 450 Β΅l)! ADP will dissolve after addition of KOH! (this is Solution A)
- 4) check pH and adjust to 7 if necessary
- 5) adjust final volume to 2 ml and divide into 0.2 ml portions
- 6) store frozen at -80 Β°C