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| |title=[[Image:Logo OROBOROS INSTRUMENTS.jpg|right|60px|link=http://www.oroboros.at|OROBOROS]] Oxygraph-2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis. | | |title=[[Image:Logo OROBOROS INSTRUMENTS.jpg|right|60px|link=http://www.oroboros.at|OROBOROS]] Oxygraph-2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis. |
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| |instruments=Oxygraph-2k, TIP2k, Fluorometry, pH, NO, TPP, Ca, Theory | | |instruments=Oxygraph-2k, TIP2k, Fluorometry, pH, NO, TPP, Ca, Theory |
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| | | REDIRECT: '''[[O2k versus multiwell respirometer]]''' |
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| » '''''More details on O2k-Specifications?'' [http://www.oroboros.at/?O2k-Specifications Consult OROBOROS]'''
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| == Discussion ==
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| Scientists who have experience with both instruments are invited to extend the discussion:
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| ::» [[Talk:MiPNet18.10_O2k_versus_multiwell]]
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| ::» [[Talk:Rogers 2011 PLoS One|XFe for isolated mitochondria - a critical discussion]]
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| == Selected related links ==
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| ::» [http://www.oroboros.at/?low-o2k-running-costs Low running costs for the OROBOROS O2k]
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| ::» [http://www.oroboros.at/?Power-O2k Power-O2k]
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| ::» [[O2k-Publications: Topics]]
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| == Conflict of interest ==
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| :: [[Gnaiger E|Erich Gnaiger]] is founder and CEO of OROBOROS INSTRUMENTS, and responsible for the development of the Oxygraph-2k in collaboration with the O2k-Team and our partners. A series of methodological publications is the result of positive feedback between his work as faculty staff of the Medical University of Innsbruck, his company initiatives and numerous international cooperations.
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| == Selected references ==
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| ::* [[Gnaiger 1995 J Bioenerg Biomembr|Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.]]
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| ::* [[Steinlechner-Maran 1996 Am J Physiol Cell Physiol|Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271: C2053-C2061.]]
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| ::* [[Gnaiger 2000 Proc Natl Acad Sci U S A|Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085.]]
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| ::* [[Gnaiger 2001 Respir Physiol|Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.]]
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| ::* [[Huetter 2004 Biochem J|Hütter E, Renner K, Pfister G, Stöckl P, Jansen-Dürr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380: 919-928.]]
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| ::* [[Kuznetsov 2004 Am J Physiol Heart Circ Physiol|Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286: H1633–H1641.]]
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| ::* [[Gnaiger 2008 POS|Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352.]]
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| ::* [[Scandurra 2010 Adv Exp Med Biol|Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25.]]
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| ::* [[Aguirre 2010 Biochim Biophys Acta|Aguirre E, Rodríguez-Juárez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797: 557-565.]]
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| ::* [[Votion 2012 PLoS One|Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890.]]
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| ::* [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]]
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| ::* [[Gnaiger 2012 MitoPathways|Gnaiger E (2012) Mitochondrial Pathways and Respiratory Control. An Introduction to OXPHOS Analysis. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp.]]
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| {{MitoPedia methods
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| |mitopedia method=Respirometry
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| |type=Respiration
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| {{MitoPedia topics|type=Respiration
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| [[Category:OroboPedia]] | | [[Category:OroboPedia]] |
| Oxygraph-2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis.
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» Open Access-MiPNet18.10.pdf ; Versions
OROBOROS (2014) Mitochondr Physiol Network
Abstract: Gnaiger E, Fasching M (2014) Oxygraph-2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis. Mitochondr Physiol Network 18.10(04): 1-8.
• Keywords: O2k-Specifications
• O2k-Network Lab: AT Innsbruck OROBOROS
Labels: MiParea: Respiration, Instruments;methods
Organism: Human
Preparation: Intact cells, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated Mitochondria"Isolated Mitochondria" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property., SMP
HRR: Oxygraph-2k, TIP2k, Fluorometry"Fluorometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., pH, NO, TPP, Ca, Theory
REDIRECT: O2k versus multiwell respirometer
