Klingenberg 1970 Eur J Biochem: Difference between revisions
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{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration | ||
|preparations=Isolated | |preparations=Isolated mitochondria | ||
|enzymes=Complex II; | |enzymes=Complex II;succinate dehydrogenase | ||
|topics=Uncoupler | |topics=Uncoupler | ||
|couplingstates=ETS | |couplingstates=ETS | ||
|substratestates= | |substratestates=CGpDH | ||
|additional=Glycerol-phosphate dehydrogenase, Uncoupler | |additional=Glycerol-phosphate dehydrogenase, Uncoupler | ||
}} | }} |
Revision as of 10:49, 13 February 2015
Klingenberg M (1970) Localization of the glycerol-phosphate dehydrogenase in the outer phase of the mitochondrial inner membrane. Eur J Biochem 13:247-52. |
ยป PMID: 5439930
Klingenberg M (1970) Eur J Biochem
Abstract: Evidence for the localization of the mitochondrial glycerol-phosphate dehydrogenase in the outer phase of the inner membrane is presented.
- Glycerol-phosphate does not permeate through the inner membrane to the matrix space in a number of mitochondria which actively oxidize glycerol-phosphate.
- The impermeability of the inner membrane to ferricyanide is demonstrated and this forms a basis for elucidating membrane localization of carriers.
- In mitochondria from various tissues ferricyanide can interact directly with glycerolphosphate dehydrogenase but not with succinate dehydrogenase.
- The oxidation of glycerol-phosphate, in contrast to that of succinate, is insensitive to substrate depletion brought about by uncouplers.
- The results are interpreted to show that both the substrate and the acceptor-site of glycerol-phosphate dehydrogenase are exposed to the outer side of the inner membrane. Thus, for the operation of the glycerol-phosphate dehydrogenase, mitochondria do not require translocases for glycerol-phosphate and dihydroxyacetone-phosphate.
Labels: MiParea: Respiration
Preparation: Isolated mitochondria
Enzyme: Complex II;succinate dehydrogenase
Regulation: Uncoupler
Coupling state: ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.
Glycerol-phosphate dehydrogenase, Uncoupler