- high-resolution terminology - matching measurements at high-resolution
Noncoupled respiration
Description
[[Description::Non-coupled respiration is distinguished from general (pharmacological or mechanical) uncoupled respiration, to give a label to an effort to reach the fully uncoupled (non-coupled) state without inhibiting respiration. Non-coupled respiration, therefore, yields an estimate of ETS capacity. Experimentally uncoupled respiration may fail to yield an estimate of ETS capacity, due to inhibition of respiration above optimum uncoupler concentrations or insufficient stimulation by sub-optimal uncoupler concentrations. Optimum uncoupler concentrations for evaluation of (non-coupled) ETS capacity require inhibitor titrations (Steinlechner-Maran_1996_AJP; Huetter_2004_BJ; Gnaiger_2008_POS).
Non-coupled respiration is maximum electron flow in an open-transmembrane proton circuit mode of operation (see ETS capacity).]]
Abbreviation: E
Reference: [[Info::MiPNet12.15; List of respiratory states]]
MitoPedia topics:
Respiratory state
Labels:
Preparation: Intact Cell; Cultured; Primary, Isolated Mitochondria, Permeabilized Cell or Tissue; Homogenate
Regulation: Respiratory state, Respiration; OXPHOS; ETS Capacity, Coupling; Membrane Potential
- Why non-coupled versus uncoupled? - Talk:Uncoupled respiration
