From Bioblast
Description
Mitochondrial respiration medium, MiR05, developed for oxygraph incubations of mitochondrial preparations. MiR06 = MiR05 + catalase. MiR05Cr = MiR05 + creatine.
Abbreviation: MiR05
Reference: MiR05-Kit, Gnaiger 2000 Life in the Cold
Comparison of respiration media
- Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42. - ยปBioblast linkยซ - The original article describing MiR05.
- Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - ยปBioblast linkยซ
- Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - ยปBioblast linkยซ - "when the goal is to maximize ADP-stimulated respiration, buffers containing K-lactobionate or K-gluconate are superior choices compared to the standard KCl-based buffers".
- Komlรณdi T, Sobotkay O, Krumschnabel G, Bezuidenhoutz N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. - ยปBioblast linkยซ
MiR05-Kit in HRR
- For preparation of MiR05, see MiR05-Kit.
- The oxygen solubility factor of MiR05 is 0.92.
MitoPedia topics:
Media for respirometry