Kanner 1976 Biochem Biophys Res Commun

Kanner BI, Serrano R, Kandrach MA, Racker E. (1976) Preparation and characterization of homogeneous coupling factor 6 from bovine heart mitochondria. Biochem Biophys Res Commun 69: 1050-1056.

» [[Has info::PMID: 132173]]

Kanner BI, Serrano R, Kandrach MA, Racker E (1976) Biochem Biophys Res Commun

Abstract: Coupling factor 6 (F6) and mitochondrial ATPase inhibitor were isolated from the rutamycin-sensitive ATPase complex of bovine heart mitochondria by heating and fractionation with ethanol. F6 appeared in acrylamide gel electrophoresis in the presence of sodium dodecylsulfate and urea as a single band corresponding to a molecular weight of 8,000. This protein which is required for the 32Pi-ATP exchange in submitochondrial particles treated with silicotungstate was very sensitive to trypsin. • Keywords: Coupling factor 6, Trypsin sensitivity, Beef heart

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Organism: Mammals  Tissue;cell: Heart  Preparation: Isolated Mitochondria 

Regulation: ATP; ADP; AMP; PCr 

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