Acin-Perez 2021 Life (Basel)

Has title::Acin-Perez R, Benincá C, Shabane B, Shirihai OS, Stiles L (2021) Utilization of human samples for assessment of mitochondrial bioenergetics: gold standards, limitations, and future perspectives. Life (Basel) 11:949. doi: 10.3390/life11090949

» [[Has info::PMID: 34575097 Open Access]]

Was written by::Acin-Perez R, Was written by::Beninca C, Was written by::Shabane B, Was written by::Shirihai OS, Was written by::Stiles L (Was published in year::2021) Was published in journal::Life (Basel)

Abstract: [[has abstract::Mitochondrial bioenergetic function is a central component of cellular metabolism in health and disease. Mitochondrial oxidative phosphorylation is critical for maintaining energetic homeostasis, and impairment of mitochondrial function underlies the development and progression of metabolic diseases and aging. However, measurement of mitochondrial bioenergetic function can be challenging in human samples due to limitations in the size of the collected sample. Furthermore, the collection of samples from human cohorts is often spread over multiple days and locations, which makes immediate sample processing and bioenergetics analysis challenging. Therefore, sample selection and choice of tests should be carefully considered. Basic research, clinical trials, and mitochondrial disease diagnosis rely primarily on skeletal muscle samples. However, obtaining skeletal muscle biopsies requires an appropriate clinical setting and specialized personnel, making skeletal muscle a less suitable tissue for certain research studies. Circulating white blood cells and platelets offer a promising primary tissue alternative to biopsies for the study of mitochondrial bioenergetics. Recent advances in frozen respirometry protocols combined with the utilization of minimally invasive and non-invasive samples may provide promise for future mitochondrial research studies in humans. Here we review the human samples commonly used for the measurement of mitochondrial bioenergetics with a focus on the advantages and limitations of each sample.]]

• Bioblast editor: has editor::Gnaiger E

Labels: MiParea: MiP area::Respiration, MiP area::mt-Medicine 

Stress:Injury and adaptation::Cryopreservation, Injury and adaptation::Mitochondrial disease  Organism: Organism::Human  Tissue;cell: tissue and cell::Skeletal muscle, tissue and cell::Blood cells, tissue and cell::Fibroblast, tissue and cell::Platelet  Preparation: Preparation::Intact organ, Preparation::Permeabilized cells, Preparation::Permeabilized tissue, Preparation::Intact cells  Enzyme: Enzyme::Complex I, Enzyme::Marker enzyme 

Coupling state: Coupling states::LEAK, Coupling states::ROUTINE, Coupling states::OXPHOS, Coupling states::ET