Agrimi 2014 Abstract IOC 2014-04 Schroecken
| Agrimi G, Mena MC, Izumi K, Pisano I, Germinario L, Fukuzaki H, Palmieri L, Blank LM, Kitagaki H (2014) Increased mitochondrial pyruvate dissimilation in sake yeast. Mitochondr Physiol Network 19.02. |
Link:
Agrimi G, Mena MC, Izumi K, Pisano I, Germinario L, Fukuzaki H, Palmieri L, Blank LM, Kitagaki H (2014)
Event: MiPNet19.02 IOC 2014-04 Schroecken
Although the decrease of pyruvate secretion by brewer’s yeasts during fermentation has long been desired in the alcohol beverage industry, rather little is known about the regulation of pyruvate accumulation. In this study, we have characterized a previously developed a pyruvate undersecreting sake yeast obtained by isolating a strain (TCR7) tolerant to ethyl α-transcyanocinnamate, an inhibitor of pyruvate transport into mitochondria. To obtain insights into pyruvate metabolism, we investigated the mitochondrial activity of TCR7 by oxigraphy and 13C-metabolic flux analysis during aerobic growth . While mitochondrial pyruvate oxidation was higher, glycerol production was decreased in TCR7 compared to the reference. These results indicate that mitochondrial activity is elevated in the TCR7 strain with the consequence of decreased pyruvate accumulation. Surprisingly mitochondrial activity is much higher in the sake yeast compared to CEN.PK 113-7D, the reference strain in metabolic engineering. When shifted from aerobic to anaerobic conditions, sake yeast retains a branched mitochondrial structure for a longer time than laboratory strains. The regulation of mitochondrial activity can become a completely novel approach to manipulate metabolic profile during fermentation of brewer’s yeasts.
• O2k-Network Lab: IT Bari Agrimi G
Labels: MiParea: Respiration, mt-Membrane
Organism: Saccharomyces cerevisiae
Preparation: Intact cells, Isolated mitochondria Enzyme: Inner mt-membrane transporter, TCA cycle and matrix dehydrogenases Regulation: Aerobic glycolysis, Flux control, Substrate; Glucose; TCA Cycle"Substrate; Glucose; TCA Cycle" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
Biotechnology
Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona 4, 70125 Bari, Italy
Department of Environmental Sciences, Faculty of Agriculture, Saga University, 1 Honjo-cho, Saga 840-8502, Japan
Institute of Applied Microbiology - iAMB, ABBt – Aachen Biology and Biotechnology Department, RWTH Aachen University, 52074 Aachen, Germany
