Beaufils 2021 J Allergy Clin Immunol
» [[Has info::PMID: 33819511 Open Access]]
Was written by::Beaufils Fabien, Was written by::Esteves Pauline, Was written by::Enaud Raphaael, Was written by::Germande Ophelie, Was written by::Celle Alexis, Was written by::Marthan Roger, Was written by::Trian Thomas, Was written by::Fayon Michael, Was written by::Berger Patrick (Was published in year::2021) Was published in journal::J Allergy Clin Immunol
Abstract: [[has abstract::Bronchial remodeling is a key feature of asthma that is already present in preschoolers with wheezing. Moreover, bronchial smooth muscle (BSM) remodeling at preschool age is predictive of asthma at school age. However, the mechanism responsible for BSM remodeling in preschoolers with wheezing remains totally unknown. In contrast, in adult asthma, BSM remodeling has been associated with an increase in BSM cell proliferation related to increased mitochondrial mass and biogenesis triggered by an altered calcium homeostasis. Indeed, BSM cell proliferation was decreased in vitro by the calcium channel blocker gallopamil.
Our aim was to investigate the mechanisms involved in BSM cell proliferation in preschoolers with severe wheezing, with special attention to the role of mitochondria and calcium signaling.
Bronchial tissue samples obtained from 12 preschool controls without wheezing and 10 preschoolers with severe wheezing were used to measure BSM mass and establish primary BSM cell cultures. BSM cell proliferation was assessed by manual counting and flow cytometry, ATP content was assessed by bioluminescence, mitochondrial respiration was assessed by using either the Seahorse or Oroboros technique, mitochondrial mass and biogenesis were assessed by immunoblotting, and calcium response to carbachol was assessed by confocal microscopy. The effect of gallopamil was also evaluated.
BSM mass, cell proliferation, ATP content, mitochondrial respiration, mass and biogenesis, and calcium response were all increased in preschoolers with severe wheezing compared with in the controls. Gallopamil significantly decreased BSM mitochondrial biogenesis and mass, as well as cell proliferation.
Mitochondria are key players in BSM cell proliferation in preschoolers with severe wheezing and could represent a potential target to treat BSM remodeling at an early stage of the disease.]] • Keywords: has publicationkeywords::Asthma, has publicationkeywords::Children, has publicationkeywords::Bronchial remodeling, has publicationkeywords::Mitochondrial biogenesis, has publicationkeywords::Calcium • Bioblast editor: [[has editor::Reiswig R]]
Labels: MiParea: MiP area::Respiration, MiP area::mt-Biogenesis;mt-density
Organism: Organism::Human
Tissue;cell: tissue and cell::Lung;gill
Preparation: Preparation::Intact cells
Coupling state: Coupling states::ROUTINE
