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* '''MitoPedia news''' - the [[uncoupler]] '''[[CCCP]]''': In our on-going experiments with mitochondria from different tissues and [[O2k-Publications:_Topics#Preparations|mt-preparations]], results on respiration and mt-membrane potential were fully comparable using CCCP or [[FCCP]] as uncouplers (protonophores) in parallel experiments. From now on our lab will replace FCCP by CCCP. Optimum concentrations for the estimation of [[ETS capacity]] are only slightly higher for CCCP compared to FCCP. Importantly, '''CCCP is far less expensive''' than FCCP (protocol development supported by ''[[MitoCom_Diagnostic_Concept|MitoCom]]''). | * '''MitoPedia news''' - the [[uncoupler]] '''[[CCCP]]''': In our on-going experiments with mitochondria from different tissues and [[O2k-Publications:_Topics#Preparations|mt-preparations]], results on respiration and mt-membrane potential were fully comparable using CCCP or [[FCCP]] as uncouplers (protonophores) in parallel experiments. From now on our lab will replace FCCP by CCCP. Optimum concentrations for the estimation of [[ETS capacity]] are only slightly higher for CCCP compared to FCCP. Importantly, '''CCCP is far less expensive''' than FCCP (protocol development supported by ''[[MitoCom_Diagnostic_Concept|MitoCom]]''). | ||
* '''Selection of appropriate methodologies''': A recently published review by Perry and colleagues provides a critical assessment of methodologies and instruments currently used in respirometry – excellent reading. [[Perry_2013_Diabetes|Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053.]] | * '''Selection of appropriate methodologies''': A recently published review by Perry and colleagues provides a critical assessment of methodologies and instruments currently used in respirometry – excellent reading. [[Perry_2013_Diabetes|Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053.]] | ||
* '''MitoPedia Glossary''' - ''new focus section'': [[MitoPedia Glossary: Respiratory control ratios|Respiratory control ratios]]. | * '''MitoPedia Glossary''' - ''new focus section'': [[MitoPedia Glossary: Respiratory control ratios|Respiratory control ratios]]. | ||
'''[[Bioblast alert]]''': for information purposes, not intended to be an advertisement. Contributions are welcome. | '''[[Bioblast alert]]''': for information purposes, not intended to be an advertisement. Contributions are welcome. | ||
Revision as of 23:14, 22 August 2013
Bioblast alert 2013(02)
- MitoPedia news - the uncoupler CCCP: In our on-going experiments with mitochondria from different tissues and mt-preparations, results on respiration and mt-membrane potential were fully comparable using CCCP or FCCP as uncouplers (protonophores) in parallel experiments. From now on our lab will replace FCCP by CCCP. Optimum concentrations for the estimation of ETS capacity are only slightly higher for CCCP compared to FCCP. Importantly, CCCP is far less expensive than FCCP (protocol development supported by MitoCom).
- Selection of appropriate methodologies: A recently published review by Perry and colleagues provides a critical assessment of methodologies and instruments currently used in respirometry – excellent reading. Perry CG, Kane DA, Lanza IR, Neufer PD (2013) Methods for assessing mitochondrial function in diabetes. Diabetes 62: 1041-1053.
- MitoPedia Glossary - new focus section: Respiratory control ratios.
Bioblast alert: for information purposes, not intended to be an advertisement. Contributions are welcome.
Power-O2k
Multiple O2k-units are offered as a ‘Power-O2k System’ of high-resolution respirometry for high-throughput with high output. Many MiPNet Laboratories have increased their high-resolution throughput by multiple O2k-units: Power-O2k
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