Bioblast quiz

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Exemplary quiz

Note: Questions in this exemplary quiz were used from a set of questions prepared for the MiPschool Tromso-Bergen 2018: The protonmotive force and respiratory control. 1. Coupling of electron transfer reactions to vectorial translocation of protons. 2. From Einstein’s diffusion equation on gradients to Fick’s law on compartments. - Gnaiger 2018 MiPschool Tromso A2

Only one correct answer.

1 Convert the molar format of the Gibbs force of reaction, Δ_kF_nO2 [kJ/mol], into the electrical format, Δ_kF_eO2 [V]. Which physicochemical constant is required?

constant

2 What is the meaning of the symbol z_O2?

	Charge number of O2 = 4
	Alphabetical order of O2 isotope
	Atomic number of O2 = 8
	Elementary charge of O2 in [C]

3 How are the units of electric energy [J] and electric force [V] related?

	V = J·C
	V = (J·F)/C
	V = J/C
	V = J/(C·F)

4 Express -460 kJ/mol O₂ as electrical force in units of volt [V].

	-1.2 V
	- 120 V
	- 1.2 kV
	1.2 V

5 Why should we do that?

	To express both in identical motive units [MU]
	To feel insecure
	To compliment our brain mitochondria
	To get free drinks

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Blue Book chapter 1: basic questions

1 The O2k-FluoRespirometer is primarily designed for which type of research?

	Comprehensive mitochondrial function assessment, including oxygen consumption
	Glycolysis rate measurement
	Quantification of mitochondrial DNA
	Measurement of mitochondrial membrane potential only

2 Peter Mitchell's chemiosmotic coupling theory places fundamental importance on what concept for bioenergetics?

	The operation of ATP synthase
	The role of cytochromes
	Bioblasts as the systematic unit
	Mitochondrial DNA's function

3 Which is NOT a parameter measured by integrating fluorometry into high-resolution respirometry?

	H2O2 production
	Mitochondrial membrane potential changes
	Glucose uptake rates
	O2 consumption rates

4 What components constitute the protonmotive force (pmF) essential for ATP synthesis in mitochondria?

	ΔΨ and ΔpH
	ΔΨ and solute concentration
	Only ΔpH
	Only ΔΨ

5 High-resolution respirometry (HRR) is primarily used for what purpose?

	pH measurement of the mitochondrial matrix
	Observing mitochondria physically
	Quantitative analysis of mitochondrial respiration and function
	Measuring cellular glucose concentration

6 Oxygen concentration impacts mitochondrial respiratory control by:

	Directly determining the rate of glycolysis
	Being inversely proportional to the rate of ATP synthesis
	Influencing exergonic and endergonic reactions in OXPHOS
	Having no significant impact on mitochondrial function

7 The statement that mitochondrial fitness "solely depends on the genetic makeup of the individual" is:

	True, but only in the context of mitochondrial diseases.
	Misleading, since mitochondrial fitness can be improved with supplements.
	Incorrect, as lifestyle and environmental factors also significantly influence mitochondrial fitness.
	True, genetics are the only factor.

8 What does the term "bioblasts" refer to in the context of mitochondrial physiology?

	Enzymes involved in the electron transport chain.
	The smallest units of DNA within mitochondria.
	Elementary units or microorganisms acting wherever living forces are present, essentially mitochondria.
	A specific type of mitochondria found in muscle cells.

9 Which of the following is NOT a direct measurement capability of the Oroboros-O2k?

	ATP production
	Protein synthesis rates
	Calcium concentration
	H2O2 production

10 What components constitute the protonmotive force (pmF) essential for ATP synthesis in mitochondria?

	Only ΔpH
	ΔΨ and solute concentration
	ΔΨ (mitochondrial membrane potential) and ΔpH
	Only ΔΨ

11 High-resolution respirometry (HRR) is primarily used for what purpose?

	Quantitative analysis of mitochondrial respiration and function
	pH measurement of the mitochondrial matrix
	Measuring cellular glucose concentration
	Observing mitochondria physically

12 Oxygen concentration impacts mitochondrial respiratory control by:

	Directly determining the rate of glycolysis
	Being inversely proportional to the rate of ATP synthesis
	Influencing exergonic and endergonic reactions in OXPHOS
	Having no significant impact on mitochondrial function

13 The "Q-junction" in mitochondrial respiratory control serves as:

	A convergence point for multiple electron transport pathways
	The site of ATP synthesis
	The mitochondrial DNA replication site
	The location where glucose is converted into pyruvate

14 SUIT protocols in mitochondrial research are designed to:

	Measure the physical size of mitochondria under different conditions
	Disrupt mitochondrial DNA and study its effects on respiration
	Identify the best culture medium for mitochondrial growth
	Analyze the effects of substrates, uncouplers, and inhibitors on respiratory control

15 NADH-linked substrates are used in physiological respiratory states to:

	Bypass the electron transport system
	Reflect the exclusive type of substrates used by mitochondria
	Demonstrate substrates irrelevant to mitochondrial physiology
	Represent substrates feeding electrons into the ETS, simulating physiological conditions

16 The primary purpose of integrating fluorometry with high-resolution respirometry is to:

	Allow for the observation of mitochondrial shape and size
	Decrease the time required for each measurement
	Enable simultaneous measurement of oxygen consumption and other mitochondrial parameters
	Increase the resolution of respirometry measurements alone

17 Which statement accurately describes the significance of LEAK respiration in the context of mitochondrial function?

	It denotes the respiration process exclusive to glycolytic cells.
	It indicates the rate of oxygen consumption for ATP synthesis.
	It is the maximum respiration rate achievable by mitochondria.
	It represents the energy consumed to maintain ionic gradients in the absence of ATP synthesis.

18 In mitochondrial research, the term "ET capacity" refers to:

	The enzyme titration capacity in metabolic pathways.
	The maximum electron transport rate through the electron transport chain under optimal conditions.
	The capacity for energy transfer within the mitochondrion.
	The ability of the endoplasmic reticulum to transfer proteins to mitochondria.

19 Which of the following is NOT a direct measurement capability of the Oroboros-O2k?

	Calcium ion concentration in the mitochondrial matrix
	ATP production rates
	Mitochondrial DNA replication rates
	Reactive oxygen species (ROS) production

20 The addition of fluorescent dyes in O2k-FluoRespirometer measurements allows for the assessment of:

	Membrane fluidity and viscosity
	Mitochondrial membrane potential changes
	The rate of glycolysis in mitochondria
	Nuclear DNA mutations

21 The primary purpose of substrate-uncoupler-inhibitor titration (SUIT) protocols in mitochondrial research is to:

	Determine the maximum capacity of the electron transport system (ETS)
	Measure the physical dimensions of mitochondria under various metabolic conditions
	Identify the optimal conditions for ATP synthesis
	Investigate the effects of different substrates, uncouplers, and inhibitors on mitochondrial respiratory control

22 I. Given the formula for protonmotive force (pmF) as Δp = Δψ - 2.303 (RT/F) (ΔpH), where Δψ is the mitochondrial membrane potential, R is the gas constant, T is temperature in Kelvin, F is Faraday's constant, and ΔpH is the pH gradient across the mitochondrial membrane. If Δψ = 150 mV, T = 310 K, and ΔpH = 1, calculate the pmF in millivolts (mV). Assume R = 8.314 J/mol·K and F = 96485 C/mol.

	Approximately 170 mV
	The pmF cannot be calculated without additional data
	Approximately 130 mV
	Approximately 220 mV

23 II. The P/O ratio is an indicator of the efficiency of ATP synthesis relative to oxygen consumption. If 10 moles of ATP are produced for every 5 moles of oxygen consumed, what is the P/O ratio? What does this imply about the mitochondrial oxidative phosphorylation efficiency?

	The P/O ratio is irrelevant to oxidative phosphorylation efficiency
	P/O = 0.5; indicates a low efficiency of oxidative phosphorylation
	P/O = 1; indicates a moderate efficiency of oxidative phosphorylation
	P/O = 2; indicates a high efficiency of oxidative phosphorylation

24 III. Assuming the standard reduction potential (E°') for NADH → NAD+ is -0.320 V and for O2 → H2O is +0.815 V, calculate the ΔE°' for the electron transport from NADH to O2. What does ΔE°' indicate about the potential energy available for ATP synthesis?

	ΔE°' = 1.135 V; indicates a high potential energy available for ATP synthesis
	ΔE°' = 0.495 V; indicates a moderate potential energy available for ATP synthesis

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