Biological contamination: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|description=Biological contamination may be caused by microbial growth in the O2k | |description=Biological contamination may be caused by microbial growth in the O2k-Chamber or in the experimental medium. | ||
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:::: Biological contamination of the O2k-Chamber is detected as a high O<sub>2</sub> flux in the [[Closed chamber |'''closed chamber''']], measured in a [[Oxygen sensor test |'''QC1: Oxygen sensor test''']] or [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background test''']]. | |||
{{Technical support}} | |||
:::: Biological contamination of the | |||
:::: The problem can be solved by first cleaning the | :::: The problem can be solved by first cleaning the O2k-Chamber by chemical sterilization (70 % ethanol with 30 % water; not 100 % ethanol) and test with a freshly prepared experimental medium, such as [[MiR05]]. Make sure the 70% ethanol does not contain any additives; ''e.g.'', 70 % ethanol used in hospital settings may contain antiseptics ([[Talk:MiPNet19.03 O2k-cleaning and ISS#Problem solved |EtOH containing antiseptics]] provided by [[Garcia-Roves PM]]). If repeated washing and storage with 70 % ethanol does not remove a biological contamination, the glass chamber has to be cleaned as described for protein contamination under [[MiPNet19.03 O2k-cleaning and ISS |O2k-Chamber cleaning with HCl]] by removing the glass chamber from the O2k and using a strong acid. |
Latest revision as of 16:29, 5 November 2020
Description
Biological contamination may be caused by microbial growth in the O2k-Chamber or in the experimental medium.
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
- Biological contamination of the O2k-Chamber is detected as a high O2 flux in the closed chamber, measured in a QC1: Oxygen sensor test or QC2: Instrumental O2 background test.
- The problem can be solved by first cleaning the O2k-Chamber by chemical sterilization (70 % ethanol with 30 % water; not 100 % ethanol) and test with a freshly prepared experimental medium, such as MiR05. Make sure the 70% ethanol does not contain any additives; e.g., 70 % ethanol used in hospital settings may contain antiseptics (EtOH containing antiseptics provided by Garcia-Roves PM). If repeated washing and storage with 70 % ethanol does not remove a biological contamination, the glass chamber has to be cleaned as described for protein contamination under O2k-Chamber cleaning with HCl by removing the glass chamber from the O2k and using a strong acid.