Difference between revisions of "Bromme 2001 Horm Metab Res"
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{{Publication | {{Publication | ||
|title=Brömme HJ, Weinandy R, Peschke D, Peschke E (2001) Simultaneous quantitative determination of alloxan, GSH, and GSSG by HPLC. Estimation of the frequency of redox cycling between alloxan and dialuric acid. Horm. Metab. Res. 33: 106-109. | |title=Brömme HJ, Weinandy R, Peschke D, Peschke E (2001) Simultaneous quantitative determination of alloxan, GSH, and GSSG by HPLC. Estimation of the frequency of redox cycling between alloxan and dialuric acid. Horm. Metab. Res. 33: 106-109. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/11294491 PMID: 11294491] | |||
|authors=Broemme HJ, Weinandy R, Peschke D, Peschke E | |authors=Broemme HJ, Weinandy R, Peschke D, Peschke E | ||
|year=2001 | |year=2001 | ||
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|abstract=This ''in vitro'' study compares the frequency of redox cycling between alloxan and dialuric acid at different initial ratios of glutathione and alloxan. Alloxan oxidizes GSH to GSSG. The rate of GSH oxidation at a given initial GSH concentration of 2.0 mmol/L depends on the initial concentration of alloxan added. The higher the concentration of alloxan in relation to the initial concentration of GSH, the faster GSH oxidation proceeds, as well as oxygen consumption, and therefore, formation of reactive oxygen species. The highest rates of GSH oxidation, i. e. GSSG formation, were found at concentration ratios of between 2.0 mmol/L GSH and 0.2 and 0.04 mmol/L alloxan, respectively. Because 0.04 mmol/L alloxan oxidizes 2.0 mmol/L GSH completely, a frequency of at least 25 cycles between alloxan and dialuric acid within 3 hours can be assumed. During each redox cycle, two molecules of GSH are oxidized to one molecule of GSSG, and during each cycle one molecule of oxygen is reduced simultaneously to one molecule of hydrogen peroxide. In total, therefore, one molecule of alloxan oxidizes at least 50 molecules of GSH and forms about 25 molecules of hydrogen peroxide. | |abstract=This ''in vitro'' study compares the frequency of redox cycling between alloxan and dialuric acid at different initial ratios of glutathione and alloxan. Alloxan oxidizes GSH to GSSG. The rate of GSH oxidation at a given initial GSH concentration of 2.0 mmol/L depends on the initial concentration of alloxan added. The higher the concentration of alloxan in relation to the initial concentration of GSH, the faster GSH oxidation proceeds, as well as oxygen consumption, and therefore, formation of reactive oxygen species. The highest rates of GSH oxidation, i. e. GSSG formation, were found at concentration ratios of between 2.0 mmol/L GSH and 0.2 and 0.04 mmol/L alloxan, respectively. Because 0.04 mmol/L alloxan oxidizes 2.0 mmol/L GSH completely, a frequency of at least 25 cycles between alloxan and dialuric acid within 3 hours can be assumed. During each redox cycle, two molecules of GSH are oxidized to one molecule of GSSG, and during each cycle one molecule of oxygen is reduced simultaneously to one molecule of hydrogen peroxide. In total, therefore, one molecule of alloxan oxidizes at least 50 molecules of GSH and forms about 25 molecules of hydrogen peroxide. | ||
|keywords=Alloxan, GSH, GSSG, HPLC, ROS | |keywords=Alloxan, GSH, GSSG, HPLC, ROS | ||
| | |discipline=Pharmacology; Biotechnology | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|instruments=Oxygraph-2k | |||
|preparations=Oxidase; Biochemical Oxidation | |||
|topics=Respiration; OXPHOS; ETS Capacity | |||
|discipline=Pharmacology; Biotechnology | |discipline=Pharmacology; Biotechnology | ||
}} | }} | ||
Revision as of 16:06, 10 November 2011
| Brömme HJ, Weinandy R, Peschke D, Peschke E (2001) Simultaneous quantitative determination of alloxan, GSH, and GSSG by HPLC. Estimation of the frequency of redox cycling between alloxan and dialuric acid. Horm. Metab. Res. 33: 106-109. |
Broemme HJ, Weinandy R, Peschke D, Peschke E (2001) Horm. Metab. Res.
Abstract: This in vitro study compares the frequency of redox cycling between alloxan and dialuric acid at different initial ratios of glutathione and alloxan. Alloxan oxidizes GSH to GSSG. The rate of GSH oxidation at a given initial GSH concentration of 2.0 mmol/L depends on the initial concentration of alloxan added. The higher the concentration of alloxan in relation to the initial concentration of GSH, the faster GSH oxidation proceeds, as well as oxygen consumption, and therefore, formation of reactive oxygen species. The highest rates of GSH oxidation, i. e. GSSG formation, were found at concentration ratios of between 2.0 mmol/L GSH and 0.2 and 0.04 mmol/L alloxan, respectively. Because 0.04 mmol/L alloxan oxidizes 2.0 mmol/L GSH completely, a frequency of at least 25 cycles between alloxan and dialuric acid within 3 hours can be assumed. During each redox cycle, two molecules of GSH are oxidized to one molecule of GSSG, and during each cycle one molecule of oxygen is reduced simultaneously to one molecule of hydrogen peroxide. In total, therefore, one molecule of alloxan oxidizes at least 50 molecules of GSH and forms about 25 molecules of hydrogen peroxide. • Keywords: Alloxan, GSH, GSSG, HPLC, ROS
Labels:
Preparation: Oxidase; Biochemical Oxidation"Oxidase; Biochemical Oxidation" is not in the list (Intact organism, Intact organ, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Chloroplasts, Enzyme, Oxidase;biochemical oxidation, ...) of allowed values for the "Preparation" property.
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
HRR: Oxygraph-2k
