Carballal 2021 J Biol Chem
| Carballal S, Vitvitsky V, Kumar R, Hanna DA, Libiad M, Gupta A, Jones JW, Banerjee R (2021) Hydrogen sulfide stimulates lipid biogenesis from glutamine that is dependent on the mitochondrial NAD(P)H pool. J Biol Chem 100950. |
Carballal Sebastian, Vitvitsky Victor, Kumar Roshan, Hanna David A, Libiad Marouane, Gupta Aditi, Jones Jace W, Banerjee Ruma (2021) J Biol Chem
Abstract: Mammalian cells synthesize H2<\sub>S from sulfur containing amino acids and are also exposed to exogenous sources of this signaling molecule, notably from gut microbes. As an inhibitor of complex IV in the electron transport chain, H2<\sub>S can have a profound impact on metabolism, suggesting the hypothesis that metabolic reprogramming is a primary mechanism by which H2<\sub>S signals. In this study, we report that H2<\sub>S increases lipogenesis in many cell types, using carbon derived from glutamine rather than from glucose. H2<\sub>S-stimulated lipid synthesis is sensitive to the mitochondrial NAD(P)H pools and is enabled by reductive carboxylation of Ξ±-ketoglutarate. Lipidomics analysis revealed that H2<\sub>S elicits time-dependent changes across several lipid classes, e.g., upregulating triglycerides while down regulating phosphatidylcholine. Direct analysis of triglyceride concentration revealed that H2<\sub>S induces a net increase in the size of this lipid pool. These results provide a mechanistic framework for understanding the effects of H2<\sub>S on increasing lipid droplets in adipocytes and population studies that have pointed to a positive correlation between cysteine (a substrate for H2<\sub>S synthesis) and fat mass.
β’ Bioblast editor: Reiswig R
Labels: MiParea: Respiration, Pharmacology;toxicology
Organism: Human
Tissue;cell: Other cell lines
Preparation: Intact cells
Regulation: Substrate
HRR: Oxygraph-2k
2021-07
