Difference between revisions of "Cardoso 2022 Abstract Bioblast"
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{{Abstract | {{Abstract | ||
|title=Cardoso | |title=4.3. '''«10+5»''' [[File:CardosoLHD.JPG|left|100px|Luiza Cardoso]] <u>Cardoso Luiza HD</u>, Doerrier C, Donnelly C, Komlódi T, Gnaiger E (2022) Redox monitoring and respiration - a new horizon with the NextGen-O2k. '''Bioblast 2022: BEC Inaugural Conference.''' In: https://doi.org/10.26124/bec:2022-0001 [[File:WatchThePresentationYoutube_icon.jpg|200px|link=https://www.youtube.com/watch?v=mNSu-hY3hUg&t=1962s|»''Watch the presentation''«]] | ||
|info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | |info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | ||
|authors=Cardoso Luiza HD, Gnaiger Erich | |authors=Cardoso Luiza HD, Doerrier Carolina, Donnelly Chris, KomlĂłdi Timea, Gnaiger Erich | ||
|year=2022 | |year=2022 | ||
|abstract=NADH-linked substrates (N-substrates) of TCA-cycle and other mt-matrix dehydrogenases feed electrons through the N-junction into Complex I. The redox states of the NAD-pool â defined as the sum of NAD(P)<sup>+</sup> and NAD(P)H â and of the electron transfer ( | |event=Bioblast 2022 | ||
 | |abstract=NADH-linked substrates (N-substrates) of TCA-cycle and other mt-matrix dehydrogenases feed electrons through the N-junction into Complex I. The redox states of the NAD-pool â defined as the sum of NAD(P)<sup>+</sup> and NAD(P)H â and of the electron transfer system (ETS)-reactive coenzyme Q-pool [1] are linked in the N-pathway but are regulated independently by several convergent electron entries into the Q-junction [2]. Our results obtained with HRR and the NADH- and Q-Modules of the NextGen-O2k show that complementary to ET-pathway control of respiratory rate and redox state (redox push by electron input), coupling control exerts opposite effects on the metabolic parameters. Whereas redox push reduces the N- and Q-pool in the LEAK state at low O<sub>2</sub> flux and high protonomotive force ''pmF'', stimulation of O<sub>2</sub> flux by ADP in the OXPHOS state [3] is accompanied by a redox pull to the oxidized state. Under these conditions, ET-pathways converging at the Q-junction yield partial additivity of O<sub>2</sub> flux, when ET-capacity downstream of Q and phosphorylation capacity exert flux control [2]. Monitoring of respiration together with NAD(P)H autofluorescence and Q-redox state [1] provides unique analytical and diagnostic power in the study of mitochondrial respiratory control at the N- and Q-junctions. | ||
# Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed | <small> | ||
# Gnaiger E et al â MitoEAGLE Task Group (2020) Mitochondrial physiology | # KomlĂłdi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https://doi.org/10.26124/bec:2021-0003 | ||
# Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. https://doi.org/10.26124/bec:2020-0002 | |||
# Gnaiger E et al â MitoEAGLE Task Group (2020) Mitochondrial physiology. https://doi.org/10.26124/bec:2020-0001.v1 | |||
</small> | |||
|mipnetlab=AT Innsbruck Oroboros | |mipnetlab=AT Innsbruck Oroboros | ||
}} | }} | ||
== Affiliations and support == | == Affiliations and support == | ||
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:::: This work was part of the Oroboros [[NextGen-O2k]] project, with funding from the European Unionâs Horizon 2020 research and innovation programme under grant agreement nÂș 859770. | :::: This work was part of the Oroboros [[NextGen-O2k]] project, with funding from the European Unionâs Horizon 2020 research and innovation programme under grant agreement nÂș 859770. | ||
 | == List of abbreviations, terms and definitions - MitoPedia == | ||
== | {{Template:List of abbreviations, terms and definitions - MitoPedia}} | ||
{{Labeling | {{Labeling | ||
|event=A4 | |||
|area=Respiration | |area=Respiration | ||
|organism=Mouse | |organism=Mouse |
Latest revision as of 08:34, 28 July 2022
4.3. «10+5» Cardoso Luiza HD, Doerrier C, Donnelly C, Komlódi T, Gnaiger E (2022) Redox monitoring and respiration - a new horizon with the NextGen-O2k. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001 |
Link: Bioblast 2022: BEC Inaugural Conference
Cardoso Luiza HD, Doerrier Carolina, Donnelly Chris, KomlĂłdi Timea, Gnaiger Erich (2022)
Event: Bioblast 2022
NADH-linked substrates (N-substrates) of TCA-cycle and other mt-matrix dehydrogenases feed electrons through the N-junction into Complex I. The redox states of the NAD-pool â defined as the sum of NAD(P)+ and NAD(P)H â and of the electron transfer system (ETS)-reactive coenzyme Q-pool [1] are linked in the N-pathway but are regulated independently by several convergent electron entries into the Q-junction [2]. Our results obtained with HRR and the NADH- and Q-Modules of the NextGen-O2k show that complementary to ET-pathway control of respiratory rate and redox state (redox push by electron input), coupling control exerts opposite effects on the metabolic parameters. Whereas redox push reduces the N- and Q-pool in the LEAK state at low O2 flux and high protonomotive force pmF, stimulation of O2 flux by ADP in the OXPHOS state [3] is accompanied by a redox pull to the oxidized state. Under these conditions, ET-pathways converging at the Q-junction yield partial additivity of O2 flux, when ET-capacity downstream of Q and phosphorylation capacity exert flux control [2]. Monitoring of respiration together with NAD(P)H autofluorescence and Q-redox state [1] provides unique analytical and diagnostic power in the study of mitochondrial respiratory control at the N- and Q-junctions.
- KomlĂłdi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. https://doi.org/10.26124/bec:2021-0003
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. https://doi.org/10.26124/bec:2020-0002
- Gnaiger E et al â MitoEAGLE Task Group (2020) Mitochondrial physiology. https://doi.org/10.26124/bec:2020-0001.v1
âą O2k-Network Lab: AT Innsbruck Oroboros
Affiliations and support
- Oroboros Instruments GmbH, Innsbruck, Austria
- This work was part of the Oroboros NextGen-O2k project, with funding from the European Unionâs Horizon 2020 research and innovation programme under grant agreement nÂș 859770.
List of abbreviations, terms and definitions - MitoPedia
Labels: MiParea: Respiration
Organism: Mouse
Tissue;cell: Liver, HEK
Preparation: Permeabilized cells, Isolated mitochondria
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, NS, ROX
HRR: Oxygraph-2k, NextGen-O2k
Event: A4