Charles 2020 Nanomedicine (Lond)

Charles C, Cohen-Erez I, Kazaoka B, Melnikov O, Stein DE, Sensenig R, Rapaport H, Orynbayeva Z (2020) Mitochondrial responses to organelle-specific drug delivering nanoparticles composed of polypeptide and peptide complexes. Nanomedicine (Lond) [Epub ahead of print].

» PMID: 33241963

Charles Carleigh, Cohen-Erez Ifat, Kazaoka Blake, Melnikov Olga, Stein David E, Sensenig Richard, Rapaport Hanna, Orynbayeva Zulfiya (2020) Nanomedicine (Lond)

Abstract: The mechanistic study of the drug carrier-target interactions of mitochondria-unique nanoparticles composed of polypeptide-peptide complexes (mPoP-NPs).

The isolated organelles were employed to address the direct effects of mPoP-NPs on dynamic structure and functional wellbeing of mitochondria. Mitochondria morphology, respiration, membrane potential, reactive oxygen species generation, were examined by confocal microscopy, flow cytometry and oxygraphy. Lonidamine-encapsulated formulation was assessed to evaluate the drug delivery capacity of the naive nanoparticles.

The mPoP-NPs do not alter mitochondria structure and performance upon docking to organelles, while successfully delivering drug that causes organelle dysfunction.

The study gives insight into interactions of mPoP-NPs with mitochondria and provides substantial support for consideration of designed nanoparticles as biocompatible and efficient mitochondria-targeted platforms. • Keywords: OxPhos, TPP+, Cancer, Ionidamine, Membrane potential, Mitochondria, Nanoparticles • Bioblast editor: Plangger M

Labels: MiParea: Respiration, mt-Structure;fission;fusion, Pharmacology;toxicology 

Organism: Human  Tissue;cell: Endothelial;epithelial;mesothelial cell  Preparation: Isolated mitochondria 

Regulation: mt-Membrane potential  Coupling state: LEAK, OXPHOS  Pathway: N, S, CIV, ROX  HRR: Oxygraph-2k, O2k-Fluorometer, TPP 

2020-12, AmR