Chinopoulos Christos

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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping

Chinopoulos Christos

MitoPedia topics: MitoPedia topic::EAGLE 

COST: Has COST::Member COST WG1: Has COST WG1::WG1

COST Mentor: Has COST Mentor::Mentor

MC Member - Management Committee MitoEAGLE

Name	Has lastname::Chinopoulos Has firstname::Christos, Has title::MD, PhD, Associate Professor
Institution	[[Has institution:: Department of Medical Biochemistry, Semmelweis University, HU]]
Address	Has address::Tuzolto st. 37-47, Has area code::1094
City	In city::Budapest
State/Province	In state::
Country	In country::Hungary
Email	Has mailaddress::[email protected]
Weblink	[[Weblink::Christos Chinopoulos]]
O2k-Network Lab	{{#ask:has member::Chinopoulos Christos}}

Bioenergetics Communications

• Christos Chinopoulos is an Executive Editor of the journal Bioenergetics Communications

Keywords: mitochondria, oncometabolism, tumor metabolism, substrate-level phosphorylation

NextGen-O2k

• Prof. Christos Chinopoulos is Key Opinion Leader (KOL) in the NextGen-O2k project.

MitoEAGLE Short-Term Scientific Mission

• • • • STSM Grant Period 2

Work Plan summary

In our laboratory we examine the contribution of mitochondrial substrate-level phosphorylation (mSLP) to ATP output and ANT directionality during true anoxia or inhibition of the electron transport chain (ETC) in isolated mitochondria or permeabilized cells. True anoxia is ensured by monitoring oxygen concentration in sealed Oroboros chambers when mitochondria respire on various substrates in the abundance of ADP. On the other hand, chemical anoxia is induced by targeted inhibition of ETC components. Under such conditions mSLP is addressed by protocols relying on membrane potential measurements using safranine O, also monitored by the O2k-Fluo LED2-Module, as described elsewhere (FASEB J. 2010, 24:2405). However, it has come to our attention that targeted inhibition of complex I perturbs mSLP even in the presence of true anoxia. Parallel to these measurements, metabolomic analysis of citricacid cycle metabolites obtained from fractions of such experiments hint on a privileged dependence of complex I activity to KGDHC. Currently, we are attempting to address this further by measuring NADH in standard fluorimetric cuvettes using home-made 3D-printed plugs in order to induce and maintain anoxia where we can also measure membrane potential by safranine O among other parameters. However, we are only partially successful, and we need to be able to measure oxygen, NADH and safranine O fluorescence in the same samples (albeit in different chambers), simultaneously. The O2k-NextGen is designed by Oroboros for achieving exactly that. The plan is to isolate mouse liver mitochondria, subject them to ADP and substrates in sealed Oroboros chambers, attain anoxia, add the ETC inhibitors and collect fractions at various time points to be evaluated by untargeted metabolomic analysis. Anoxia, NADH levels and membrane potential will be measured throughout these experiments and complement the results of the metabolomic analysis.

Participated at

• NextGen-O2k Summit 2021 Virtual
• IOC141 Schroecken AT
• MitoEAGLE 2018 Innsbruck AT
• MitoEAGLE 2018 Jurmala LV
• IOC127 Szeged HU
• MitoFit Workshop ATP 2017 Innsbruck AT
• IOC116 Innsbruck AT
• Bioblast 2012 Innsbruck AT
• IOC66 Innsbruck AT
• IOC127 Szeged HU

Visiting scientist in the Oroboros O2k-Laboratory

Christos Chinopoulos : Visiting scientist at the Oroboros O2k-Laboratory

• December 14 to December 22 2017

Labels: Field of research: field of research::Basic  Topics: topics::Mitochondrial mechanisms of neurodegeneration, topics::Permeability transition pore 

Publications

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Abstracts

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