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: [[Management_Committee_MitoEAGLE#MC_Members|MC Member]] - [[Management Committee MitoEAGLE]] | |||
{{Person | {{Person | ||
|lastname=Velika | |lastname=Čižmárová (Velika) | ||
|firstname=Beata | |firstname=Beata | ||
|title=PhD, RNDr. | |title=PhD, RNDr. | ||
| institution=[[File: | |institution=:::[[File:VelikaBeata.JPG|right|200px|Beata Cizmarova]] | ||
Department of Medical and Clinical Biochemistry | |||
'''Pavol Jozef Šafárik University in Kosice''' | |||
:: Faculty of Medicine | |||
:: Department of Medical and Clinical Biochemistry | |||
'''Visiting scientist''' | |||
:* Research on: Cryopreservation and respiration of blood cells | |||
Beata Cizmarova joined [[Oroboros_Contact |Oroboros]] in January 2017 within the framework | |||
<br/> of the [http://www.aktion.saia.sk/de Postdoc Scholarship Action Austria] and COST Action [[MitoEAGLE]]. | |||
|address=Trieda SNP 1 | |address=Trieda SNP 1 | ||
|area code=040 11 | |area code=040 11 | ||
|city=Košice | |city=Košice | ||
|country=Slovakia | |country=Slovakia | ||
|mailaddress= | |mailaddress=beata.cizmarova@upjs.sk | ||
}} | }} | ||
{{Labelingperson | {{Labelingperson}} | ||
}} | |||
== | |||
****: [[Short- | == MitoEAGLE Short-Term Scientific Mission == | ||
::: ''' | ****: [[Short-Term_Scientific_Missions_MitoEAGLE#STSM_Grant_Period_2 |STSM Grant Period 2]] | ||
:::: My name is | ::: '''Work Plan summary''' | ||
:::: My name is Beata Velika and I work as Research specialist at the Pavol Jozef Safarik University in Kosice, Department of Medical and Clinical Biochemistry, Faculty of Medicine, Slovakia. During my study and work I specialized in fluorescence spectroscopy, and I have experience with different isolation methods, including mitochondria isolation, and measuring of their native fluorescence and respiration. | |||
:::: | :::: I would like to carry out the project in cooperation with the research group at the Mitochondrial Research Laboratory of Prof. Dr. Erich Gnaiger in Innsbruck, Austria (Medical University of Innsbruck; K-Regio project MitoFit) in collaboration with the scientific company OROBOROS INSTRUMENTS Corp. The laboratory of Prof. Dr. Erich Gnaiger contains very high standard laboratory equipment, with several specialists in mitochondrial physiology field. The short-term project is focused on the optimization of SUIT (substrate-uncoupler-inhibitor titration) protocols for high-resolution respirometry of intact cryopreserved human peripheral blood mononuclear cells (PBMC). The usage of human PBMC will provide a significant stimulus for the entire field of mitochondrial medicine, because the mitochondrial function of PBMC is linked with numerous diseases and metabolic conditions in the human body, and the protocols developed during the project will lead to the standardization of the procedures, and should be used for studying mitochondria physiological and pathological mechanisms. Cryopreserved cells (stored at -80 °C), will be thawed in 37 °C water bath, dilluted in 10 ml pre-warmed 37 °C medium composed of DPBS + 2%FBS and centrifuged (300 g, 6 minutes). The sediment will be resuspended and counted. The suspension of cryopreserved cells will be titrated into the 2-ml chambers of the Oxygraph-2k (Oroboros Instruments, Innsbruck, Austria). We will optimize the SUIT protocols with defined substrate combinations, uncoupler and inhibitor titrations, which will be applied for the measurement of respiration of the cryopreserved cells. The results will be compared with the respiration of the fresh cells (reference). Obtained results will be presented in 10th MiPschool 2017 MitoEAGLE and MitoEAGLE Workshop WG1-4, Obergurgl AT (2017 Jul 23-30). | ||
== MitoEAGLE Inclusiveness Target Countries - Conference Grant == | |||
:::: | ****: [[MitoEAGLE_Inclusiveness_Target_Countries#Conference_Grant_-_Grand_Period_2|Conference Grant Grand Period 2]] | ||
::: '''[[Media:Velika_ITC_conference_grant_scientific_report.pdf|Scientific report - MiP2017]]''' | |||
::: '''[[Media:Velika_ITC_conference_grant_scientific_report_WISE.pdf|Scientific report - WISe3]]''' | |||
== Participated at == | |||
::::*[[MiP2018/MitoEAGLE Jurmala LV|MitoEAGLE 2018 Jurmala LV]] | |||
::::* [[MitoEAGLE Lund 2018| MitoEAGLE 2018 Lund SE]] | |||
::::* [[MiP2017/MitoEAGLE Hradec Kralove CZ|MitoEAGLE 2017 Hradec Kralove CZ]] | |||
::::* [[IOC124|IOC124 Schroecken AT]] | |||
::::* [[MiPschool Obergurgl 2017| MiPschool 2017 Obergurgl AT]] | |||
::::* [[MitoFit_Open_Seminar_2017-07-14| MitoFit Open Seminar 2017 Innsbruck AT]] | |||
::::* [[IOC122|IOC122 Schroecken AT]] | |||
::::* [[MitoEAGLE Barcelona 2017| MitoEAGLE 2017 Barelona ES]] | |||
== Visiting scientist in the Oroboros O2k-Laboratory == | |||
::: | :::: [[Image:O2k-Network.png|left|40px|link=O2k-Network|O2k-Network]] | ||
[[Cizmarova Beata| Beata Cizmarova ]]: Visiting scientist at the [[Oroboros Laboratories: visiting scientists |Oroboros O2k-Laboratory]] | |||
::::::* 2018, January 08 to 19 | |||
::::::* 2017, January 15 to July 31 |
Latest revision as of 13:19, 16 March 2020
News and Events | Working Groups | Short-Term Scientific Missions | Management Committee | Members |
COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
Cizmarova Beata
MitoPedia topics: MitoPedia topic::EAGLE
COST: Has COST::Member COST WG1: Has COST WG1::WG1
COST WG4: Has COST WG4::WG4
COST ECI: Has COST ECI::ECI
Name | Has lastname::Čižmárová (Velika) Has firstname::Beata, Has title::PhD, RNDr. |
---|---|
Institution | [[Has institution:::::
Pavol Jozef Šafárik University in Kosice
Visiting scientist
Beata Cizmarova joined Oroboros in January 2017 within the framework
|
Address | Has address::Trieda SNP 1, Has area code::040 11 |
City | In city::Košice |
State/Province | In state:: |
Country | In country::Slovakia |
Has mailaddress::[email protected] | |
Weblink | Weblink:: |
O2k-Network Lab | {{#ask:has member::Cizmarova Beata}} |
Labels:
Publications
{{#ask:was written by::Cizmarova Beata|format=table|sort=was published in year|order=desc|default=Add references to your publications
| ?was published in year=Published
| ?has title=Reference
| limit=2000
}}
Abstracts
{{#ask:was written by::Cizmarova Beata|format=table|sort=was submitted in year|order=desc|default=Add abstract
| ?was submitted in year=Published
| ?has title=Reference
| limit=2000
}}
MitoEAGLE Short-Term Scientific Mission
- Work Plan summary
- My name is Beata Velika and I work as Research specialist at the Pavol Jozef Safarik University in Kosice, Department of Medical and Clinical Biochemistry, Faculty of Medicine, Slovakia. During my study and work I specialized in fluorescence spectroscopy, and I have experience with different isolation methods, including mitochondria isolation, and measuring of their native fluorescence and respiration.
- I would like to carry out the project in cooperation with the research group at the Mitochondrial Research Laboratory of Prof. Dr. Erich Gnaiger in Innsbruck, Austria (Medical University of Innsbruck; K-Regio project MitoFit) in collaboration with the scientific company OROBOROS INSTRUMENTS Corp. The laboratory of Prof. Dr. Erich Gnaiger contains very high standard laboratory equipment, with several specialists in mitochondrial physiology field. The short-term project is focused on the optimization of SUIT (substrate-uncoupler-inhibitor titration) protocols for high-resolution respirometry of intact cryopreserved human peripheral blood mononuclear cells (PBMC). The usage of human PBMC will provide a significant stimulus for the entire field of mitochondrial medicine, because the mitochondrial function of PBMC is linked with numerous diseases and metabolic conditions in the human body, and the protocols developed during the project will lead to the standardization of the procedures, and should be used for studying mitochondria physiological and pathological mechanisms. Cryopreserved cells (stored at -80 °C), will be thawed in 37 °C water bath, dilluted in 10 ml pre-warmed 37 °C medium composed of DPBS + 2%FBS and centrifuged (300 g, 6 minutes). The sediment will be resuspended and counted. The suspension of cryopreserved cells will be titrated into the 2-ml chambers of the Oxygraph-2k (Oroboros Instruments, Innsbruck, Austria). We will optimize the SUIT protocols with defined substrate combinations, uncoupler and inhibitor titrations, which will be applied for the measurement of respiration of the cryopreserved cells. The results will be compared with the respiration of the fresh cells (reference). Obtained results will be presented in 10th MiPschool 2017 MitoEAGLE and MitoEAGLE Workshop WG1-4, Obergurgl AT (2017 Jul 23-30).
- Work Plan summary
MitoEAGLE Inclusiveness Target Countries - Conference Grant
Participated at
Visiting scientist in the Oroboros O2k-Laboratory
Beata Cizmarova : Visiting scientist at the Oroboros O2k-Laboratory
- 2018, January 08 to 19
- 2017, January 15 to July 31