Colleoni 2012 Placenta: Difference between revisions
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|abstract=Mitochondrial function is required to support energetically-demanding processes in the placenta. As such, a compromise in mitochondrial function could severely impact fetal growth and development. Respirometry is a highly useful method for studying mitochondrial function, but is not possible in freeze-thawed mitochondria, which become uncoupled. We have developed a novel method that permits respiratory analysis of cryopreserved placental tissue. We studied mitochondrial function in 7 normal human placentas, analysing both fresh and cryopreserved samples. We found no impairments in respiration following cryopreservation in the delivery suite, with enhanced coupling, as indicated by higher respiratory control ratios, than in fresh placental samples transported to the laboratory on ice. | |abstract=Mitochondrial function is required to support energetically-demanding processes in the placenta. As such, a compromise in mitochondrial function could severely impact fetal growth and development. Respirometry is a highly useful method for studying mitochondrial function, but is not possible in freeze-thawed mitochondria, which become uncoupled. We have developed a novel method that permits respiratory analysis of cryopreserved placental tissue. We studied mitochondrial function in 7 normal human placentas, analysing both fresh and cryopreserved samples. We found no impairments in respiration following cryopreservation in the delivery suite, with enhanced coupling, as indicated by higher respiratory control ratios, than in fresh placental samples transported to the laboratory on ice. | ||
|keywords=Placenta | |keywords=Placenta | ||
|mipnetlab=UK Cambridge Murray AJ | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration | ||
|injuries=Cryopreservation | |||
|organism=Human | |organism=Human | ||
|tissues=Genital | |tissues=Genital | ||
|preparations=Permeabilized tissue | |preparations=Permeabilized tissue | ||
|couplingstates=LEAK, OXPHOS | |couplingstates=LEAK, OXPHOS | ||
|pathways=N, ROX | |pathways=N, ROX | ||
}} | }} |
Latest revision as of 12:11, 27 March 2018
Colleoni F, Morash AJ, Ashmore T, Monk M, Burton GJ, Murray AJ (2012) Cryopreservation of placental biopsies for mitochondrial respiratory analysis. Placenta 33:122-3. |
ยป PMID: 22154690, Open Access
Colleoni F, Morash AJ, Ashmore T, Monk M, Burton GJ, Murray AJ (2012) Placenta
Abstract: Mitochondrial function is required to support energetically-demanding processes in the placenta. As such, a compromise in mitochondrial function could severely impact fetal growth and development. Respirometry is a highly useful method for studying mitochondrial function, but is not possible in freeze-thawed mitochondria, which become uncoupled. We have developed a novel method that permits respiratory analysis of cryopreserved placental tissue. We studied mitochondrial function in 7 normal human placentas, analysing both fresh and cryopreserved samples. We found no impairments in respiration following cryopreservation in the delivery suite, with enhanced coupling, as indicated by higher respiratory control ratios, than in fresh placental samples transported to the laboratory on ice. โข Keywords: Placenta
โข O2k-Network Lab: UK Cambridge Murray AJ
Labels: MiParea: Respiration
Stress:Cryopreservation Organism: Human Tissue;cell: Genital Preparation: Permeabilized tissue
Coupling state: LEAK, OXPHOS
Pathway: N, ROX