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Difference between revisions of "Conceicao 2021 Sci Rep"

From Bioblast
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|area=Respiration
|preparations=Intact cells
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|instruments=Oxygraph-2k
|instruments=Oxygraph-2k
|additional=2021-10
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Revision as of 17:33, 14 October 2021

Publications in the MiPMap
Conceição CC, da Silva JN, Arcanjo A, Nogueira CL, de Abreu LA, de Oliveira PL, Gondim KC, Moraes B, de Carvalho SS, da Silva RM, da Silva Vaz I Jr, Moreira LA, Logullo C (2021) Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis. Sci Rep 11:19202.

» PMID: 34584163 Open Access

Conceicao Christiano Calixto, Nascimento da Silva Jhenifer, Arcanjo Angelica, Lopes Nogueira Cintia, de Abreu Leonardo Araujo, de Oliveira Pedro Lagerbald, Gondim Katia C, Moraes Bruno, de Carvalho Stephanie Serafim, da Silva Renato Martins, da Silva Vaz Jr Itabajara, Moreira Luciano Andrade, Logullo Carlos (2021) Sci Rep

Abstract: In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate the symbiont-host interaction in the context of energy metabolism regulation and molecular mechanisms of the immune responses involved. The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells. Additionally, innate immunity mechanisms were activated, showing that the wAflu1 and wAflu1.tet cells are responsive after the stimulus using Gram negative bacteria. Therefore, this work confirms the natural, mutually co-regulating symbiotic relationship between W. pipientis and A. fluviatilis, modulating the host metabolism and immune pathway activation. The results presented here add important resources to the current knowledge of Wolbachia-arthropod interactions.

Bioblast editor: Plangger M


Labels: MiParea: Respiration 



Preparation: Intact cells 


Coupling state: LEAK, ROUTINE, ET  Pathway: ROX  HRR: Oxygraph-2k 

2021-10