Difference between revisions of "Coupling-control protocol"
| Line 1: | Line 1: | ||
{{MitoPedia | {{MitoPedia | ||
|abbr=CCP | |abbr=CCP | ||
|description=A '''coupling control protocol''' induces different [[coupling control state]]s at constant substrate supply. In [[intact cell]]s, CCP can be appled by using membrane-permeable inhibitors of the [[phosphorylation system]] (e.g. [[oligomycin]]) and [[uncoupler]]s (e.g. [[FCCP]]). | |description=A '''coupling control protocol''' induces different [[coupling control state]]s at constant substrate supply. In [[intact cell]]s, CCP can be appled by using membrane-permeable inhibitors of the [[phosphorylation system]] (e.g. [[oligomycin]]) and [[uncoupler]]s (e.g. [[FCCP]]). Coupling control states in intact cells include ''R'', ''L'', ''E''; [[LEAK]], [[ROUTINE]], and [[ETS]]. Coupling control states in isolated mitochondria, permeabilized cells or homogenates include ''L'', ''P'', ''E''; LEAK, [[OXPHOS]], and ETS. | ||
|info=Pesta_2011_Protocols | |info=Pesta_2011_Protocols | ||
|type=Respiration | |type=Respiration | ||
Revision as of 17:51, 15 May 2011
- high-resolution terminology - matching measurements at high-resolution
Coupling-control protocol
Description
A coupling control protocol induces different coupling control states at constant substrate supply. In intact cells, CCP can be appled by using membrane-permeable inhibitors of the phosphorylation system (e.g. oligomycin) and uncouplers (e.g. FCCP). Coupling control states in intact cells include R, L, E; LEAK, ROUTINE, and ETS. Coupling control states in isolated mitochondria, permeabilized cells or homogenates include L, P, E; LEAK, OXPHOS, and ETS.
Abbreviation: CCP
Reference: Pesta_2011_Protocols
Labels:
Regulation: Respiration; OXPHOS; ETS Capacity"Respiration; OXPHOS; ETS Capacity" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property.
