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Coupling-control protocol

Description

A coupling control protocol, CCP, induces different coupling control states at constant substrate supply. In intact cells, the CCP can be applied by using membrane-permeable inhibitors of the phosphorylation system (e.g. oligomycin) and uncouplers (e.g. CCCP). Coupling control states in intact cells include R, L, E; LEAK, ROUTINE, and ET-pathway. Coupling control states in isolated mitochondria, permeabilized cells or homogenates include L, P, E; LEAK, OXPHOS, and ET-pathway. Residual oxygen consumption (ROX) is finally evaluated for ROX correction of flux. The CCP may be extended, when further respiratory states (e.g. cell viability test; CIV assay) are added to the coupling control module consisting of three coupling control states. The term phosphorylation control protocol, PCP, has been introduced synonymous for CCP. » MiPNet article

Abbreviation: CCP

Reference: Gnaiger 2008 POS, Gnaiger 2014 MitoPathways

MitoPedia concepts: SUIT concept

MitoPedia methods: Respirometry

From PCP to CCP

Gnaiger E (2015) From PCP to CCP. Mitochondr Physiol Network 2015-01-11.

OROBOROS (2015) MiPNet

Abstract: The control of oxidative phosphorylation by coupling is of primary importance in OXPHOS analysis. To avoid misunderstandings, it is suggested to replace the term 'phosphorylation control protocol' by coupling control protocol, CCP.

• O2k-Network Lab: AT Innsbruck Gnaiger E

Labels:

Regulation: Coupling efficiency;uncoupling Coupling state: LEAK, ROUTINE, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.

HRR: Theory

In functional OXPHOS analysis, the control of oxidative phosphorylation by coupling is of primary importance, as studied by application of protocols, in which the phosphorylation system is either inhibited (lack of ADP; inhibition by oligomycin), activated (saturating ADP; activation by phyiological control in intact cells) or eliminated (uncoupling). It seems thus appropriate to use the term phosphorylation control protocol, PCP ^[1]. In this context it is taken for granted that we do not refer to metabolic control by phosphorylation of enzymes, which is an important mechanism to change specific enzyme activity. To avoid any such confusion, it is suggested to replace the term 'phosphorylation control protocol' by coupling control protocol, CCP^[2]

- - - : this is the coupling control protocol with intact cells (ROUTINE - LEAK_Omy - ET-pathway - ROX).

- - - : this is the comparable coupling control protocol with mitochondrial preparations (OXPHOS - LEAK_Omy - ET-pathway - ROX).

- - - - - : In mitochondrial preparations, various variations are possible for the coupling control protocol, for example in isolated mitochondria ^[3]: (LEAK_N - OXPHOS - LEAK_T - OXPHOS - ET-pathway - ROX).^[4]

References

↑ Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access«
↑ Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »Open Access«
↑ Hand SC, Gnaiger E (2014) Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11(06):1-5. »Open Access«
↑ LEAK respiration

Biochemical coupling efficiency: from 0 to <1

More details: ET-pathway coupling efficiency

O2k-Publications: coupling control protocol with intact cells

» References

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O2k-Abstracts: coupling control protocol with intact cells

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[1] Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access«

[2] Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »Open Access«

[3] Hand SC, Gnaiger E (2014) Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11(06):1-5. »Open Access«

[4] LEAK respiration

[1]

[2]

[3]

[4]

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=CCP
-|description=A '''coupling control protocol, CCP''', induces different [[coupling control state]]s at constant substrate supply. In [[Intact_cells| intact cell]]s, the CCP can be applied by using membrane-permeable inhibitors of the [[phosphorylation system]] (e.g. [[oligomycin]]) and [[uncoupler]]s (e.g. [[CCCP]]). Coupling control states in intact cells include ''R'', ''L'', ''E''; [[LEAK]], [[ROUTINE]], and [[ETS]]. Coupling control states in isolated mitochondria, permeabilized cells or homogenates include ''L'', ''P'', ''E''; LEAK, [[OXPHOS]], and ETS. [[Residual oxygen consumption]] (ROX) is finally evaluated for ROX correction of flux. The CCP may be extended, when further respiratory states (e.g. cell viability test; CIV assay) are added to the coupling control module consisting of three coupling control states. The term '''phosphorylation control protocol''', PCP, has been introduced synonymous for CCP.
+|description=A '''coupling control protocol, CCP''', induces different [[coupling control state]]s at constant substrate supply. In [[Intact_cells| intact cell]]s, the CCP can be applied by using membrane-permeable inhibitors of the [[phosphorylation system]] (e.g. [[oligomycin]]) and [[uncoupler]]s (e.g. [[CCCP]]). Coupling control states in intact cells include ''R'', ''L'', ''E''; [[LEAK]], [[ROUTINE]], and [[ET-pathway]]. Coupling control states in isolated mitochondria, permeabilized cells or homogenates include ''L'', ''P'', ''E''; LEAK, [[OXPHOS]], and ET-pathway. [[Residual oxygen consumption]] (ROX) is finally evaluated for ROX correction of flux. The CCP may be extended, when further respiratory states (e.g. cell viability test; CIV assay) are added to the coupling control module consisting of three coupling control states. The term '''phosphorylation control protocol''', PCP, has been introduced synonymous for CCP.
 » [[Coupling_control_protocol#From_PCP_to_CCP |'''MiPNet article''']]
 |info=[[Gnaiger 2008 POS]], [[Gnaiger 2014 MitoPathways]]
@@ Line 30: / Line 30: @@
-[[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] - [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:E.jpg|link=ETS capacity|ETS]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]: this is the coupling control protocol with intact cells (ROUTINE - LEAK<sub>Omy</sub> - ETS - ROX).
+[[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] - [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:E.jpg|link=ETS capacity|ET capacity]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]: this is the coupling control protocol with intact cells (ROUTINE - LEAK<sub>Omy</sub> - ET-pathway - ROX).
-[[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] - [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:E.jpg|link=ETS capacity|ETS]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]: this is the comparable coupling control protocol with [[mitochondrial preparations]] (OXPHOS - LEAK<sub>Omy</sub> - ETS - ROX).
+[[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] - [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:E.jpg|link=ETS capacity|ET capacity]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]: this is the comparable coupling control protocol with [[mitochondrial preparations]] (OXPHOS - LEAK<sub>Omy</sub> - ET-pathway - ROX).
-[[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] - [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] - [[Image:E.jpg|link=ETS capacity|ETS]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]: In mitochondrial preparations, various variations are possible for the coupling control protocol, for example in isolated mitochondria
+[[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] - [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] - [[Image:E.jpg|link=ETS capacity|ET capacity]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]: In mitochondrial preparations, various variations are possible for the coupling control protocol, for example in isolated mitochondria
-<ref> Hand SC, Gnaiger E (2014) Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11(06):1-5. »[[MiPNet12.11 MitoRespiration |Open Access]]« </ref>: (LEAK<sub>N</sub> - OXPHOS - LEAK<sub>T</sub> - OXPHOS - ETS - ROX).<ref> [[LEAK respiration]] </ref>
+<ref> Hand SC, Gnaiger E (2014) Flux control ratios in isolatd mitochondria. OXPHOS capacity and respiratory control in isolated mitochondria. Mitochondr Physiol Network 12.11(06):1-5. »[[MiPNet12.11 MitoRespiration |Open Access]]« </ref>: (LEAK<sub>N</sub> - OXPHOS - LEAK<sub>T</sub> - OXPHOS - ET-pathway - ROX).<ref> [[LEAK respiration]] </ref>
 '''References'''
@@ Line 41: / Line 41: @@
 = Biochemical coupling efficiency: from 0 to <1 =
-::::* ''More details:'' [[ETS coupling efficiency]]
+::::* ''More details:'' [[ET-pathway coupling efficiency]]
@@ Line 65: / Line 65: @@
 |order=descending
 }}
+{{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Coupling states::ROUTINE]] [[Coupling states::LEAK]] [[Coupling states::ET-pathway]]
+|?Was published in year=Year
+|?Has title=Reference
+|?Tissue and cell=Tissues; cells
+|?Mammal and model=Mammals; models
+|format=broadtable
+|limit=5000
+|offset=0
+|sort=Was published in year
+|order=descending
+}}
 [[Image:O2k-Publications.png|left|115px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]]
@@ Line 70: / Line 83: @@
   '''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''.
 {{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Coupling states::ROUTINE]] [[Coupling states::LEAK]] [[Coupling states::ETS]]
+|?Was submitted in year=Year
+|?Has title=Reference
+|?Tissue and cell=Tissues; cells
+|?Mammal and model=Mammals; models
+|format=broadtable
+|limit=5000
+|offset=0
+|sort=Was submitted in year
+|order=descending
+}}
+{{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Coupling states::ROUTINE]] [[Coupling states::LEAK]] [[Coupling states::ET-pathway]]
 |?Was submitted in year=Year
 |?Has title=Reference

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