Difference between revisions of "Coupling-control state"

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high-resolution terminology - matching measurements at high-resolution

Coupling-control state

Description

Coupling control states are defined in mitochondrial preparations (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as LEAK, OXPHOS, and ET-pathway states, with corresponding respiration rates (L, P, E) in any electron transfer-pathway state which is competent for electron transfer. These coupling states are induced by application of specific inhibitors of the phosphorylation system, titration of ADP and uncouplers. In intact cells, the coupling control states are LEAK, ROUTINE, and ET-pathway states of respiration (L, R, E). Coupling control protocols induce these coupling control states sequentially at a constant electron transfer-pathway state.

Abbreviation: CCS

Reference: Gnaiger 2009 Int J Biochem Cell Biol

The Blue Book 2014: Fig. 2.4.

Coupling control states: -

Communicated by Gnaiger E 2011-02-25, edited 2016-10-20.

MitoPedia concepts: MiP concept, Respiratory state, SUIT concept, SUIT state, Recommended

MitoPedia methods: Respirometry

MitoPedia topics: EAGLE

@@ Line 1: / Line 1: @@
 {{MitoPedia
 |abbr=''CCS''
-|description='''Coupling control states''' are defined in [[mitochondrial preparations]] (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as [[LEAK]], [[OXPHOS]], and [[ET-pathway]] states of respiration (''L, P, E'') in any [[Electron transfer-pathway state]] which is competent for electron transfer. These coupling states are induced by application of specific inhibitors of the [[phosphorylation system]], titration of ADP and uncouplers. In intact cells, the coupling control states are [[LEAK]], [[ROUTINE]], and [[ET-pathway]] states of respiration (''L, R, E''). [[Coupling control protocol]]s induce these coupling control states sequentially at a constant [[Electron transfer-pathway state]].
+|description='''Coupling control states''' are defined in [[mitochondrial preparations]] (isolated mitochondria, permeabilized cells, permeabilized tissues, homogenates) as [[LEAK]], [[OXPHOS]], and [[ET-pathway]] states, with corresponding respiration rates (''L, P, E'') in any [[electron transfer-pathway state]] which is competent for electron transfer. These coupling states are induced by application of specific inhibitors of the [[phosphorylation system]], titration of ADP and uncouplers. In intact cells, the coupling control states are [[LEAK]], [[ROUTINE]], and [[ET-pathway]] states of respiration (''L, R, E''). [[Coupling control protocol]]s induce these coupling control states sequentially at a constant [[electron transfer-pathway state]].
 |info=[[Gnaiger 2009 Int J Biochem Cell Biol]]
 }}
+[[File:EPL-free and excess.jpg|right|300px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014: Fig. 2.4.]]]]
+'''Coupling control states:''' [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET-capacity|ET-capacity]] [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
+ Communicated by [[Gnaiger E]] 2011-02-25, edited 2016-10-20.
 {{MitoPedia concepts
 |mitopedia concept=MiP concept, Respiratory state, SUIT concept, SUIT state, Recommended
@@ Line 13: / Line 18: @@
 |mitopedia topic=EAGLE
 }}
-[[File:EPL-free and excess.jpg|right|300px|thumb|[[Gnaiger 2014 MitoPathways |The Blue Book 2014: Fig. 2.4.]]]]
-'''Coupling control states:''' [[Image:P.jpg|link=OXPHOS capacity|OXPHOS]] [[Image:R.jpg|link=ROUTINE respiration|ROUTINE]] [[Image:E.jpg|link=ET-capacity|ET-capacity]] [[Image:L.jpg|link=LEAK respiration|LEAK]] - [[Image:ROX.jpg|link=Residual oxygen consumption|ROX]]
- Communicated by [[Gnaiger E]] 2011-02-25, edited 2016-10-20.