Cytochrome c: Difference between revisions
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== Application in [[HRR]]: storage and stock solution of ''c'' == | == Application in [[HRR]]: storage and stock solution of ''c'' == | ||
:::: '''''c'': Cytochrome ''c'' ''' (from equine heart), Sigma C 7752, 50 mg, store at -20 ยฐC; FW = | :::: '''''c'': Cytochrome ''c'' ''' (from equine heart), Sigma C 7752, 50 mg, store at -20 ยฐC; FW = 12384 Da. | ||
Revision as of 16:10, 25 August 2017
Description
Cytochrome c is a component of the electron transfer system (ETS) in mitochondria. It is a small heme protein loosely associated with the outer side of the inner mitochondrial membrane. The heme group of cytochrome c transfers electrons from Complex III to Complex IV. The release of cytochrome c into the cytoplasm is associated with apoptosis.
Abbreviation: c
Reference: MiPNet09.12; Gnaiger 2002 Biochem Soc Trans
MitoPedia topics: Substrate and metabolite
Application in HRR: storage and stock solution of c
- c: Cytochrome c (from equine heart), Sigma C 7752, 50 mg, store at -20 ยฐC; FW = 12384 Da.
- Preparation of 4 mM cytochrome c solution (dissolved in H2O):
- Weigh 50 mg cytochrome c into a small glass beaker. Difficult to weigh, since the powder is electromagnetic.
- Add 1 ml H2O; c dissolves easily.
- Divide into 0.2 ml portions.
- Store at -20 ยฐC.
- Caution: Chemicals stored in the fridge or freezer should be allowed to reach room temperature before opening.
- O2k manual titrations: MiPNet09.12 O2k-Titrations
- Titration volume: 5 ยตl using a 25 ยตl syringe (2 ml O2k-chamber).
- Final concentration: 10 ยตM.
Cytochrome c control: a test for outer mt-membrane integrity
- More details: ยป Cytochrome c control factor