Difference between revisions of "Drahota 2005 Cell Biochem Biophys"
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{{Publication | {{Publication | ||
|title=Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Development of cytochrome-c oxidase in rat heart. Cell Biochem Biophys 43: 87-94. | |title=Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Development of cytochrome-c oxidase in rat heart. Cell Biochem Biophys 43:87-94. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/16043886 PMID: 16043886] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/16043886 PMID: 16043886] | ||
|authors=Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B | |authors=Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B | ||
|year=2005 | |year=2005 | ||
|journal=Cell | |journal=Cell Biochem Biophys | ||
|abstract=Cytochrome | |abstract=Cytochrome ''c'' oxidase (COX) activity of the rat heart was two- to sevenfold activated when the membrane integrity was disrupted by hypotonic swelling, freezing-thawing, or a detergent, indicating that a large portion of COX capacity in intact mitochondria is not active. The effect of detergent was tested in heart mitochondria isolated from 1-, 5-, 15-, 29-, and 60-d-old rats; activation by detergent was up to 20-fold in 1-d-old animals, whereas in mitochondria from 60-d-old rats it was only 7-fold. Our data indicate that the rat heart exhibits significant developmental changes dependent on downregulation of COX activity in the neonatal period. | ||
|keywords=Rat heart, Postnatal development, Cytochrome-c oxidase, Mitochondria, Detergent | |keywords=Rat heart, Postnatal development, Cytochrome-c oxidase, Mitochondria, Detergent | ||
|mipnetlab=CZ Prague Houstek J | |||
|discipline=Biomedicine | |discipline=Biomedicine | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration | |||
|organism=Rat | |||
|tissues=Heart | |||
|preparations=Isolated mitochondria, Oxidase;biochemical oxidation | |||
|enzymes=Complex IV;cytochrome c oxidase | |||
|couplingstates=OXPHOS | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|discipline=Biomedicine | |discipline=Biomedicine | ||
}} | }} | ||
Latest revision as of 11:09, 13 February 2015
| Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Development of cytochrome-c oxidase in rat heart. Cell Biochem Biophys 43:87-94. |
Drahota Z, Milerova M, Stieglerova A, Skarka L, Houstek J, Ostadal B (2005) Cell Biochem Biophys
Abstract: Cytochrome c oxidase (COX) activity of the rat heart was two- to sevenfold activated when the membrane integrity was disrupted by hypotonic swelling, freezing-thawing, or a detergent, indicating that a large portion of COX capacity in intact mitochondria is not active. The effect of detergent was tested in heart mitochondria isolated from 1-, 5-, 15-, 29-, and 60-d-old rats; activation by detergent was up to 20-fold in 1-d-old animals, whereas in mitochondria from 60-d-old rats it was only 7-fold. Our data indicate that the rat heart exhibits significant developmental changes dependent on downregulation of COX activity in the neonatal period. • Keywords: Rat heart, Postnatal development, Cytochrome-c oxidase, Mitochondria, Detergent
• O2k-Network Lab: CZ Prague Houstek J
Labels: MiParea: Respiration
Organism: Rat
Tissue;cell: Heart
Preparation: Isolated mitochondria, Oxidase;biochemical oxidation
Enzyme: Complex IV;cytochrome c oxidase
Coupling state: OXPHOS
HRR: Oxygraph-2k
