Espino 2016 Abstract Mito Xmas Meeting Innsbruck: Difference between revisions
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{{Abstract | {{Abstract | ||
|title= | |title=Mitochondrial [Ca2+] measurements using a novel very-low Ca2+ affinity aequorin-based probe. | ||
|authors= | |authors=Espino J, De Stefani D, Rizzuto R | ||
|year=2016 | |year=2016 | ||
|event=Mito Xmas Meeting 2016 Innsbruck AT | |event=Mito Xmas Meeting 2016 Innsbruck AT | ||
|abstract= | |abstract=Aequorin is a 22-kDa photoprotein produced by the jellyfish Aequorea victoria that has been long utilised for the study of Ca2+ signaling (1). It has been also engineered to induce its specific targeting to various cell regions so as to monitor [Ca2+] in different subcellular comparments, e.g., mitochondrial matrix (2). Nevertheless, its potential applicability is somewhat limited owing to consumption or saturation of aequorin throughout the experiment as well as stability of aequorin at physiological temperature. Herein, in an attempt to overcome the aforementioned disadvantages, we have developed a mitochondria-targeted triple-mutated form (Asp119Ala, Gln168Arg and Leu170Ile) of the photoprotein aequorin that enables measurement of [Ca2+] in the millimolar range. In fact, it is shown that addition of extramitochondrial Ca2+ to permeabilized HeLa cells triggers an increase in mitochondrial [Ca2+] up to approximately 2 mM. In intact cells, the novel probe allows recording agonist-stimulated mitochondrial [Ca2+] rises without problems derived from aequorin saturation and/or consumption. Notably, in addition to the increased dynamic range, the Gln168Arg and Leu170Ile mutations endowed this new aequorin-based probe with an increase lifetime at 37°C. This also allowed the generation of a cell line stably expressing the probe at very high levels. | ||
}} | }} | ||
{{Labeling | {{Labeling | ||
|event=Poster | |||
}} | }} | ||
== Affiliations == | == Affiliations == | ||
:::: | :::: Espino J, De Stefani D, Rizzuto R | ||
::::# Dept Biomedical Sc, Univ Padova, Italy | |||
::::# | ==Reference== | ||
::::# Shimomura, O., Johnson, F.H. & Saiga, Y. (1962) Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J Cell Comp Physiol; 59: 223–239. | |||
::::# Bonora, M., Giorgi, C., Bononi, A., Marchi, S., Patergnani, S., Rimessi, A., Rizzuto, R. & Pinton, P. (2013) Subcellular calcium measurements in mammalian cells using jellyfish photoprotein aequorin-based probes. Nat Protoc; 8: 2015-2018. | |||
Revision as of 12:22, 7 December 2016
| Mitochondrial [Ca2+] measurements using a novel very-low Ca2+ affinity aequorin-based probe. |
Link:
Espino J, De Stefani D, Rizzuto R (2016)
Event: Mito Xmas Meeting 2016 Innsbruck AT
Aequorin is a 22-kDa photoprotein produced by the jellyfish Aequorea victoria that has been long utilised for the study of Ca2+ signaling (1). It has been also engineered to induce its specific targeting to various cell regions so as to monitor [Ca2+] in different subcellular comparments, e.g., mitochondrial matrix (2). Nevertheless, its potential applicability is somewhat limited owing to consumption or saturation of aequorin throughout the experiment as well as stability of aequorin at physiological temperature. Herein, in an attempt to overcome the aforementioned disadvantages, we have developed a mitochondria-targeted triple-mutated form (Asp119Ala, Gln168Arg and Leu170Ile) of the photoprotein aequorin that enables measurement of [Ca2+] in the millimolar range. In fact, it is shown that addition of extramitochondrial Ca2+ to permeabilized HeLa cells triggers an increase in mitochondrial [Ca2+] up to approximately 2 mM. In intact cells, the novel probe allows recording agonist-stimulated mitochondrial [Ca2+] rises without problems derived from aequorin saturation and/or consumption. Notably, in addition to the increased dynamic range, the Gln168Arg and Leu170Ile mutations endowed this new aequorin-based probe with an increase lifetime at 37°C. This also allowed the generation of a cell line stably expressing the probe at very high levels.
Labels:
Event: Poster
Affiliations
- Espino J, De Stefani D, Rizzuto R
- Dept Biomedical Sc, Univ Padova, Italy
Reference
- Shimomura, O., Johnson, F.H. & Saiga, Y. (1962) Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J Cell Comp Physiol; 59: 223–239.
- Bonora, M., Giorgi, C., Bononi, A., Marchi, S., Patergnani, S., Rimessi, A., Rizzuto, R. & Pinton, P. (2013) Subcellular calcium measurements in mammalian cells using jellyfish photoprotein aequorin-based probes. Nat Protoc; 8: 2015-2018.
