Fluorometric dyes: Difference between revisions

Latest revision as of 14:28, 8 February 2016

high-resolution terminology - matching measurements at high-resolution

Fluorometric dyes

Description

Extrinsic fluorophores; fluorescent markers.

MitoPedia methods: Fluorometry

Fluorometric dyes for direct quantification

ROS (H₂O₂): Amplex red
Ca2+ - Dye: Fura2
Ca2+ - Dye: Indo1
Ca2+ - Dye: Calcium green
mt-Membrane potential: Safranin
mt-Membrane potential: TMRM
mt-Membrane potential, H₂O₂: Rhodamin 123
pH: Fluorescin
pH: HPTS
Mg2+: Magnesium organge, Magnesium green
ROS (HOO-.): MitoSOX

Fluorometric dyes for ratiometric excitation

Ca2+: Fura2
mt-Membrane potential: TMRM (?)
pH: SNAFL-1
Redox state: roGFP

Fluorometric dyes for ratiometric emmission

Ca2+: Indo1
mt-Membrane potential: JC1

Autofluorescence

NADH

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-{{MitoPedia}}
+{{MitoPedia
+|description=[[Extrinsic fluorophores]]; fluorescent markers.
+}}
+{{MitoPedia concepts}}
 {{MitoPedia methods
-|mitopedia method=Fluorimetry
+|mitopedia method=Fluorometry
 }}
+{{MitoPedia O2k and high-resolution respirometry}}
 {{MitoPedia topics}}
-==Fluorimetric dyes for direct quantification==
+== Fluorometric dyes for direct quantification ==
-*ROS (H2O2) - Dye: Amplex Red
+*ROS (H<sub>2</sub>O<sub>2</sub>): [[Amplex red]]
-*Ca2+ - Dye: Fura2
+*Ca2+ - Dye: [[Fura2]]
-*Ca2+ - Dye: Indo1
+*Ca2+ - Dye: [[Indo1]]
 *Ca2+ - Dye: Calcium green
-*mt-Membrane potential - Dye: Safranin
+*mt-Membrane potential: [[Safranin]]
-*mt-Membrane potential - Dye: TMRM
+*mt-Membrane potential: [[TMRM]]
-*mt-Membrane potential, H2O2 - Dye: Rhodamin 123
+*mt-Membrane potential, H<sub>2</sub>O<sub>2</sub>: [[Rhodamin 123]]
-*pH - Dye: Fluorescin
+*pH: [[Fluorescin]]
-*pH - Dye: HPTS
+*pH: [[HPTS]]
-*Mg2+ - Dye: Magnesium organge, Magnesium green
+*Mg2+: [[Magnesium organge]], [[Magnesium green]]
-*ROS (HOO-.) - Dye: MitoSOX
+*ROS (HOO-.): [[MitoSOX]]
-==Fluorimetric dyes for ratiometric excitation==
-*Ca2+ - Dye: Fura2
-*mt-Membrane potential - Dye: TMRM (?)
-*pH - Dye: SNAFL-1
-*Redox state - Dye: roGFP
-==Fluorimetric dyes for ratiometric emmission==
-*Ca2+ - Dye: Indo1
-*mt-Membrane potential - Dye: JC1
-==Autofluorescence==
+== Fluorometric dyes for ratiometric excitation ==
-*NADH
+*Ca2+: [[Fura2]]
+*mt-Membrane potential: [[TMRM]] (?)
+*pH: [[SNAFL-1]]
+*Redox state: [[roGFP]]
+== Fluorometric dyes for ratiometric emmission ==
+*Ca2+: [[Indo1]]
+*mt-Membrane potential: [[JC1]]
-{{#set: Scientific service =Fluorescence}}
+== Autofluorescence ==
-__SHOWFACTBOX__
+*[[NADH]]
-{{Scientific service}}