Difference between revisions of "Fromme 2021 Abstract Kalorimetrietage"
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{{Abstract | {{Abstract | ||
|info=[https://www.kalorimetrietage.ptb.de/die-24-kalorimetrietage-2021] | |title=Fromme T (2021) Application of different respirometric assay systems to characterize the membrane permeabilization potency of saponins. Kalorimetrietage 2021. | ||
|info=[https://www.kalorimetrietage.ptb.de/die-24-kalorimetrietage-2021 24th Kalorimetrietage 2021 Braunschweig DE] | |||
|authors=Fromme T | |authors=Fromme T | ||
|year=2021 | |year=2021 | ||
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|mipnetlab=DE Freising Klingenspor M | |mipnetlab=DE Freising Klingenspor M | ||
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== Affiliation == | |||
:::: Tobias Fromme | |||
:::: Chair of Molecular Nutritional Medicine, Technical University Munich (TUM) School of Life Sciences, Munich, Germany | |||
{{Labeling | {{Labeling | ||
|area=Respiration | |area=Respiration | ||
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|additional=Saponin | |additional=Saponin | ||
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Revision as of 07:27, 27 May 2021
| Fromme T (2021) Application of different respirometric assay systems to characterize the membrane permeabilization potency of saponins. Kalorimetrietage 2021. |
Link: 24th Kalorimetrietage 2021 Braunschweig DE
Fromme T (2021)
Event: 24th Kalorimetrietage 2021 Braunschweig DE
Saponins are a diverse class of secondary plant metabolites, some of which known for their ability to permeabilize biological membranes. We characterized the potency of several pure saponins to permeabilize plasma membrane and mitochondrial membranes of cultured, human cells for different metabolites. An initial screen made use of the advantages of a multiplate-format flux analyzer (Agilent Seahorse) while detailed validation exploited the strengths of a high-resolution respirometer (Oroboros O2k). This study highlights specific strengths and potential synergy of methodologically different cellular respirometry approaches.
• Bioblast editor: Gnaiger E
• O2k-Network Lab: DE Freising Klingenspor M
Affiliation
- Tobias Fromme
- Chair of Molecular Nutritional Medicine, Technical University Munich (TUM) School of Life Sciences, Munich, Germany
Labels: MiParea: Respiration
Preparation: Permeabilized cells
HRR: Oxygraph-2k
Saponin
