From Bioblast
Description
Glucose, also known as D-glucose or dextrose, is a monosaccharide and an important carbohydrate in biology. Cells use it as the primary source of energy and a metabolic intermediate.
Abbreviation: Glc
Reference: MiPNet15.09. Yeast: HRR Reference Assay
Application in HRR
- Glc: Glucose (D-(+)-Glucose, C6H1206); Sigma G 8270, 100 g, store at RT; FW = 180.16
- Preparation of 2 M stock solution (dissolved in H2O):
- weigh 360.32 mg of Glucose
- dissolve 1 mL H2O in 1.5 mL Eppendorf
- Β»O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 20 Β΅L using a 50 Β΅L Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 5 Β΅L using a 25 Β΅L Hamilton syringe.
- Final concentration: 20 mM
- Preparation of 2.2 M stock solution (dissolved in H2O):
- weigh 79.2 mg of Glucose
- dissolve 0.2 mL H2O in 0.5 mL Eppendorf
- Β»O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 5 Β΅L using a 25 Β΅L Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 1.25 Β΅L using a 10 Β΅L Hamilton syringe.
- Final concentration: 5.5 mM (1 g/L)
- Β»O2k manual titrations MiPNet09.12 O2k-Titrations
- Titration volume (2-mL O2k-chamber): 10 Β΅L using a 25 Β΅L Hamilton syringe.
- Titration volume (0.5-mL O2k-chamber): 2.5 Β΅L using a 10 Β΅L Hamilton syringe.
- Final concentration: 11 mM (2 g/L)
MitoPedia topics: Substrate and metabolite