Klepinin 2016 J Bioenerg Biomembr
» [[Has info::PMID: 27854030]]
Was written by::Klepinin A, Was written by::Ounpuu L, Was written by::Guzun R, Was written by::Chekulayev V, Was written by::Timohhina N, Was written by::Tepp K, Was written by::Shevchuk I, Was written by::Schlattner U, Was written by::Kaambre T (Was published in year::2016) Was published in journal::J Bioenerg Biomembr
Abstract: [[has abstract::The adenylate kinase (AK) isoforms network plays an important role in the intracellular energy transfer processes, the maintenance of energy homeostasis, and it is a major player in AMP metabolic signaling circuits in some highly-differentiated cells. For this purpose, a rapid and sensitive method was developed that enables to estimate directly and semi-quantitatively the distribution between cytosolic AK1 and mitochondrial AK2 localized in the intermembrane space, both in isolated cells and tissue samples (biopsy material). Experiments were performed on isolated rat mitochondria or permeabilized material, including undifferentiated and differentiated neuroblastoma Neuro-2a cells, HL-1 cells, isolated rat heart cardiomyocytes as well as on human breast cancer postoperative samples. In these samples, the presence of AK1 and AK2 could be detected by high-resolution respirometry due to the functional coupling of these enzymes with ATP synthesis. By eliminating extra-mitochondrial ADP with an excess of pyruvate kinase and its substrate phosphoenolpyruvate, the coupling of the AK reaction with mitochondrial ATP synthesis could be quantified for total AK and mitochondrial AK2 as a specific AK index. In contrast to the creatine kinase pathway, the AK phosphotransfer pathway is up-regulated in murine neuroblastoma and HL-1 sarcoma cells and in these malignant cells expression of AK2 is higher than AK1. Differentiated Neuro-2a neuroblastoma cells exhibited considerably higher OXPHOS capacity than undifferentiated cells, and this was associated with a remarkable decrease in their AK activity. The respirometric method also revealed a considerable difference in mitochondrial affinity for AMP between non-transformed cells and tumor cells.]] • Keywords: has publicationkeywords::Adenylate kinase, has publicationkeywords::Breast cancer, has publicationkeywords::Cardiomyocytes, has publicationkeywords::Mitochondria, has publicationkeywords::Neuroblastoma, has publicationkeywords::Mouse neuroblastoma Neuro-2A cells, has publicationkeywords::Mouse cardiac sarcoma HL-1 cell
• O2k-Network Lab: Was published by MiPNetLab::EE Tallinn Kaambre T, Was published by MiPNetLab::EE Tallinn Kaambre T, Was published by MiPNetLab::EE Tallinn Saks VA, Was published by MiPNetLab::FR Grenoble Schlattner U
Labels: MiParea: MiP area::Respiration, MiP area::Comparative MiP;environmental MiP
Pathology: Diseases::Cancer
Organism: Organism::Human, Organism::Mouse, Organism::Rat Tissue;cell: tissue and cell::Heart, tissue and cell::Nervous system, tissue and cell::Endothelial;epithelial;mesothelial cell, tissue and cell::Genital Preparation: Preparation::Permeabilized cells, Preparation::Permeabilized tissue, Preparation::Isolated mitochondria
Regulation: Topic::ATP, Topic::AMP Coupling state: Coupling states::OXPHOS Pathway: Pathways::N, Pathways::NS HRR: Instrument and method::Oxygraph-2k
